Endotoxin upregulates expression of an antimicrobial peptide gene in mammalian airway epithelial cells.
Endotoxin upregulates expression of an antimicrobial peptide gene in mammalian airway epithelial cells.
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内毒素上调哺乳动物气道上皮细胞中抗菌肽基因的表达。
DOI：
10.1378/chest.105.3_supplement.51s
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发表时间：
1994
期刊：
影响因子：
9.6
通讯作者：
C. Bevins
中科院分区：
文献类型：
--
作者：
G. Diamond;C. Bevins
药物，通常在严重肝损伤的情况下。后者表明，PIM可能会在某些情况下诱导，我们设计了这些实验，以诱导吞噬肺单核细胞在一个物种，他们通常不普遍。本研究的目的是确定是否可以在大鼠（通常没有这些细胞的物种）中诱导PIM，以及这种诱导的单核细胞对病原体的肺摄取是否与肺损伤相关。大鼠静脉注射生理盐水（n=4）或3 mg/kg大肠杆菌0111：B4脂多糖（LPS）（n=12）。一天后，所有动物静脉注射示踪颗粒，5 mg/kg磁性氧化铁（直径0.5 μ m）。1小时后，人道处死动物，并使用1 g组织样品中的磁场强度测定肺、肝和其他内脏中氧化铁的吞噬摄取。第二组生理盐水对照组（n=6）和LPS注射组（n=6）大鼠在24 h时注射2 x 10 μ菌落形成单位/kg活铜绿假单胞菌，以确定循环病原颗粒的命运。通过器官样品的定量培养来确定1小时的吞噬摄取。将每组动物的肺和肝样品固定用于超微结构研究，以确定负责氧化铁和假单胞菌摄取的细胞类型。以氧化铁或细菌回收剂量的平均百分比± SEM表示的器官分布为：
maceuticals, usually in cases of severe liver damage. The latter suggests that PIMs may be induced under certain circumstances, and we have designed these experiments to induce phagocytic pulmonary mononuclear cells in a species where they are not normally prevalent. The aims of this study were to determine whether PIMs can be induced in rats, a species normally without these cells, and whether pulmonary uptake of pathogens by such induced mononuclear cells is associated with lung injury. Rats were injected intravenously with saline solution (n=4) or 3 mg/kg Escherichia coli 0111:B4 hipopolysaccharide (LPS) (n=12). One day later, all animals were injected intravenously with a tracer particle, 5 mg/kg magnetic iron oxide (0.5 p.m diameter). One hour later, animals were humanely killed and phagocytic uptake of iron oxide in lungs, liver, and other internal organs was determined using magnetic field strength in 1 g tissue samples. A second group of saline solution control (n=6) and LPS-injected (n=6) rats was injected at 24 h with 2 x 10� colony-forming units/kg live Pseudomonas aernginosa to determine the fate of a circulating pathogenic particle. Phagocytic uptake at 1 h was determined by quantitative culture of organ samples. Lung and liver samples from each group of animals were fixed for ultrastructural study to determine the cell types responsible for iron oxide and Pseudomonas uptake. Organ distribution as the mean percent of recovered dose of iron oxide or bacteria ± SEM were:
绵羊肺血管内巨噬细胞摄取血源性细菌以及随后的炎症反应。
DOI：
10.1164/ajrccm/136.3.683
发表时间：
1987
期刊：
The American review of respiratory disease
影响因子：
--
作者：
Warner,AE;Molina,RM;Brain,JD
通讯作者：
Brain,JD