Characteristics of repair tissue in second-look and third-look biopsies from patients treated with engineered cartilage: relationship to symptomatology and time after implantation.
Characteristics of repair tissue in second-look and third-look biopsies from patients treated with engineered cartilage: relationship to symptomatology and time after implantation.
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DOI：
10.1186/ar2549
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发表时间：
2008
影响因子：
4.9
通讯作者：
Abatangelo G
中科院分区：
文献类型：
--
作者：
Brun P;Dickinson SC;Zavan B;Cortivo R;Hollander AP;Abatangelo G
目前的研究建立了组织再生的特点，在患者患有膝关节病变的治疗与移植的自体软骨细胞生长在三维透明质酸生物材料。这项多中心研究涉及植入后5 - 33个月的二次关节镜检查/活检（n = 63）。7例患者允许进行第三次活检，其中3例在植入后18个月进行。组织学和组织化学的特点进行了评价。评价剩余的骨残端的软骨/骨整合。为了进行数据分析，活检进一步分为来自术后有症状患者（n = 41）或来自无症状患者（n = 22）的活检。植入后18个月内获得的活检中透明再生组织的百分比显着高于植入后18个月内获得的活检。在有症状和无症状患者之间也观察到差异：移植后18个月从有症状患者中取出的修复组织主要是纤维软骨或混合（透明-纤维软骨）组织，而从无症状患者中取出的组织在83%的活检中是透明软骨。在一小组无症状患者（n = 3）中，术后18个月进行的第二次和第三次活检证实了新形成的组织随着时间的推移而成熟。从与软骨下骨接触的移植物内部区域开始，向植入物外围发生软骨成熟。该研究表明，在软骨细胞植入后的无症状患者中，再生组织经历了一个成熟过程，在大多数情况下需要超过18个月的时间才能完成，并导致透明组织而不是纤维软骨。症状的持续可能反映了非透明软骨修复组织的存在。
The present study established characteristics of tissue regrowth in patients suffering knee lesions treated with grafts of autologous chondrocytes grown on three-dimensional hyaluronic acid biomaterials. This multicentred study involved a second-look arthroscopy/biopsy, 5 to 33 months post implant (n = 63). Seven patients allowed a third-look biopsy, three of which were performed 18 months post implant. Characteristics of tissues were histologically and histochemically evaluated. The remaining bone stubs were evaluated for cartilage/bone integration. For data analysis, biopsies were further divided into those obtained from postoperative symptomatic patients (n = 41) or from asymptomatic patients (n = 22). The percentage of hyaline regenerated tissues was significantly greater in biopsies obtained after, versus within, 18 months of implantation. Differences were also observed between symptomatic and asymptomatic patients: reparative tissues taken from symptomatic patients 18 months after grafting were mainly fibrocartilage or mixed (hyaline–fibrocartilage) tissue, while tissues taken from asymptomatic patients were hyaline cartilage in 83% of biopsies. In a small group of asymptomatic patients (n = 3), second-look and third-look biopsies taken 18 months after surgery confirmed maturation of the newly formed tissue over time. Cartilage maturation occurred from the inner regions of the graft, in contact with subchondral bone, towards the periphery of the implant. The study indicates that, in asymptomatic patients after chondrocyte implantation, regenerated tissue undergoes a process of maturation that in the majority of cases takes longer than 18 months for completion and leads to hyaline tissue and not fibrous cartilage. Persistence of symptoms might reflect the presence of a nonhyaline cartilage repair tissue.
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影响因子：
7
作者：
Tognana, E;Chen, F;Freed, LE
通讯作者：
Freed, LE
DOI：
10.1097/01.blo.0000165737.87628.5b
发表时间：
2005-06-01
影响因子：
4.2
作者：
Marcacci, M;Berruto, M;Zanasi, S
通讯作者：
Zanasi, S
或者，剪接的II型Procollagen mRNA定义了椎骨发育过程中的不同细胞种群：氨基甲基肽的差异表达。
DOI：
10.1083/jcb.114.6.1307
发表时间：
1991-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Sandell LJ;Morris N;Robbins JR;Goldring MB
通讯作者：
Goldring MB
影响因子：
--
作者：
Dickinson, SC;Sims, TJ;Hollander, AP
通讯作者：
Hollander, AP
DOI：
10.1097/00003086-200005000-00020
发表时间：
2000-05-01
影响因子：
4.2
作者：
Peterson, L;Minas, T;Lindahl, A
通讯作者：
Lindahl, A