CC chemokine ligand 18 correlates with malignant progression of prostate cancer.
CC chemokine ligand 18 correlates with malignant progression of prostate cancer.
复制标题
CC趋化因子配体18与前列腺癌的恶性进展相关
DOI：
10.1155/2014/230183
复制
发表时间：
2014
影响因子：
--
通讯作者：
Zhong WD
中科院分区：
文献类型：
--
作者：
Chen G;Liang YX;Zhu JG;Fu X;Chen YF;Mo RJ;Zhou L;Fu H;Bi XC;He HC;Yang SB;Wu YD;Jiang FN;Zhong WD
背景和目标。CC趋化因子配体18（CCL18）促进各种人类癌症类型的恶性行为。然而，其在人类前列腺癌中的参与尚未完全阐明。本研究的目的是探讨CCL18在PCa中的作用。方法.采用实时定量PCR和免疫组织化学分析检测CCL18在mRNA和蛋白水平的表达。我们分析了CCL18表达与人PCa临床特征的相关性。检测PCa细胞迁移、侵袭和凋亡的影响。在皮下异种移植模型中评估CCL18对前列腺肿瘤生长的效率。结果CCL18在PCa组织中的表达明显高于非癌组织（P均< 0.01）。CCL18上调与前列腺癌患者的高Gleason评分相关（P = 0.034）。rCCL18刺激PCa细胞可促进细胞迁移和侵袭，但可降低DU 145细胞的凋亡率。CCL18刺激小鼠皮下移植瘤模型显示肿瘤生长和肿瘤血管化增加，Ki 67、PCNA和CD 31的表达也显著增加。结论.我们的数据提供了令人信服的证据表明，CCL18的上调可能参与PCa的恶性进展。
Background and Aim. CC chemokine ligand 18 (CCL18) promotes malignant behaviors of various human cancer types. However, its involvement in human prostate cancer has not been fully elucidated. The aim of this study was to investigate the role of CCL18 in PCa. Methods. Expression of CCL18 at mRNA and protein levels was detected using real-time qRT-PCR and immunohistochemistry analysis. We analyzed the associations of CCL18 expression with clinical features of human PCa. The effects of PCa cell migration, invasion, and apoptosis were tested. The efficiency of CCL18 on prostate tumor growth was assessed in a subcutaneous xenograft model. Results. CCL18 expression was upregulated (both P < 0.01) in PCa tissues compared with those in noncancerous prostate tissues. CCL18 upregulation was correlated with high Gleason score (P = 0.034) of patients with PCa. rCCL18 stimulation in PCa cells promoted cell migration and invasion but decreased DU145 cells apoptosis rate. Furthermore, subcutaneous homografts models showed the increased tumor growth and tumor vascularization with the CCL18 stimulation, and the expression of Ki67, PCNA, and CD31 in CCL18 stimulation mice was also significantly increased. Conclusions. Our data offer the convincing evidence that the upregulation of CCL18 may be involved in the malignant progression of PCa.
登录
查看更多内容
DOI：
10.1111/cea.12032
发表时间：
2013-02
期刊：
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
影响因子：
--
作者：
Gavala ML;Kelly EA;Esnault S;Kukreja S;Evans MD;Bertics PJ;Chupp GL;Jarjour NN
通讯作者：
Jarjour NN
影响因子：
1.6
作者：
Molitierno, Joseph;Evans, Aubrey;Pruthi, Raj S.
通讯作者：
Pruthi, Raj S.
影响因子：
254.7
作者：
Siegel, Rebecca;Naishadham, Deepa;Jemal, Ahmedin
通讯作者：
Jemal, Ahmedin
影响因子：
3.4
作者：
Pittet MJ
通讯作者：
Pittet MJ
影响因子：
158.5
作者：
Eisenberger, MA;Blumenstein, BA;Lowe, BA
通讯作者：
Lowe, BA