Nalidixic acid-resistant mutations of the gyrB gene of Escherichia coli
Nalidixic acid-resistant mutations of the gyrB gene of Escherichia coli
复制标题
大肠杆菌gyrB基因的萘啶酸抗性突变
DOI：
10.1007/bf00331012
复制
发表时间：
1986
期刊：
影响因子：
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通讯作者：
Shinichi Nakamura
中科院分区：
文献类型：
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作者：
J. Yamagishi;H. Yoshida;M. Yamayoshi;Shinichi Nakamura
从大肠杆菌KL-16和自发耐萘啶酸突变体中克隆了含有gyrB基因的3.4kb的DNA片段。通过转导分析确定突变（nal-24和nal-31）在gyrB基因中。对克隆的DNA片段的核苷酸序列分析表明，nal-24是gyrB基因第426位密码子的第一个碱基处的G到A的转换，导致氨基酸从天冬氨酸变为天冬酰胺，nal-31是第447位密码子的第一个碱基处的A到G的转换，导致氨基酸从赖氨酸变为谷氨酸。这表明gyrB基因的突变是萘啶酸耐药性的原因。
SummaryDNA fragments of 3.4 kb containing the gyrB gene were cloned from Escherichia coli KL-16 and from spontaneous nalidixic acid-resistant mutants. The mutations (nal-24 and nal-31) had been determined to be in the gyrB gene by transduction analysis. Nucleotide sequence analysis of the cloned DNA fragments revealed that nal-24 was a G to A transition at the first base of the 426th codon of the gyrB gene, resulting in an amino acid change from aspartic acid to asparagine, and nal-31 was an A to G transition at the first base of the 447th codon, resulting in an amino acid change from lysine to glutamic acid. This indicates that mutations in the gyrB gene are responsible for nalidixic acid resistance.
DOI：
10.1073/pnas.81.15.4622
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
BLANAR, MA;SANDLER, SJ;CLARK, AJ
通讯作者：
CLARK, AJ