Gelam Honey Inhibits the Production of Proinflammatory, Mediators NO, PGE(2), TNF-α, and IL-6 in Carrageenan-Induced Acute Paw Edema in Rats.
Gelam Honey Inhibits the Production of Proinflammatory, Mediators NO, PGE(2), TNF-α, and IL-6 in Carrageenan-Induced Acute Paw Edema in Rats.
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DOI：
10.1155/2012/109636
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Mohd Yusof YA
中科院分区：
文献类型：
--
作者：
Hussein SZ;Mohd Yusoff K;Makpol S;Mohd Yusof YA
天然蜂蜜以其治疗价值而闻名，并已被用于世界各地不同文化的传统医学。本研究的目的是探讨马来西亚Gelam蜂蜜在炎症诱导大鼠中的抗炎作用。通过将1%角叉菜胶足底下注射到大鼠右后爪中来诱导爪水肿。将大鼠用非留体抗炎药（NSAID）吲哚美辛（10mg/kg，p.o.）或不同剂量的Gelam蜂蜜（1或2g/kg，p.o.）。足垫厚度增加被认为是水肿，使用千分表测量。收集血浆和爪组织以分析炎症介质如NO、PGE 2、TNF-α和IL-6以及iNOS和考克斯-2的产生。结果表明，Gelam蜂蜜能剂量依赖性地减轻炎症大鼠足爪水肿，降低血浆NO、PGE 2、TNF-α和IL-6的产生，抑制足爪组织iNOS、考克斯-2、TNF-α和IL-6的表达。在两个时间点（1天和7天）以2g/kg体重口服预处理Gelam蜂蜜显示促炎细胞因子的产生显著降低，这与抗炎药物吲哚美辛（NSAID）在血浆和组织中的作用相似。因此，我们的研究结果表明，Gelam蜂蜜具有抗炎作用，通过减少大鼠足肿胀的大小和抑制促炎介质的产生。Gelam蜂蜜对治疗炎症有潜在的作用。
Natural honey is well known for its therapeutic value and has been used in traditional medicine of different cultures throughout the world. The aim of this study was to investigate the anti-inflammatory effect of Malaysian Gelam honey in inflammation-induced rats. Paw edema was induced by a subplantar injection of 1% carrageenan into the rat right hind paw. Rats were treated with the nonsteroidal anti-inflammatory drug (NSAID) Indomethacin (10 mg/kg, p.o.) or Gelam honey at different doses (1 or 2 g/kg, p.o.). The increase in footpad thickness was considered to be edema, which was measured using a dial caliper. Plasma and paw tissue were collected to analyze the production of inflammatory mediators, such as NO, PGE2, TNF-α, and IL-6, as well as iNOS and COX-2. The results showed that Gelam honey could reduce edema in a dose-dependent fashion in inflamed rat paws, decrease the production of NO, PGE2, TNF-α, and IL-6 in plasma, and suppress the expression of iNOS, COX-2, TNF-α, and IL-6 in paw tissue. Oral pretreatment of Gelam honey at 2 g/kg of body weight at two time points (1 and 7 days) showed a significantly decreased production of proinflammatory cytokines, which was similar to the effect of the anti-inflammatory drug Indomethacin (NSAID), both in plasma and tissue. Thus, our results suggest that Gelam honey has anti-inflammatory effects by reducing the rat paw edema size and inhibiting the production of proinflammatory mediators. Gelam honey is potentially useful for treating inflammatory conditions.
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影响因子：
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作者：
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