Novel distance-progesterone-combined selection approach improves human sperm quality.
Novel distance-progesterone-combined selection approach improves human sperm quality.
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新颖的距离-黄体酮组合选择方法可提高人类精子质量
DOI：
10.1186/s12967-018-1575-7
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发表时间：
2018-07-20
影响因子：
7.4
通讯作者：
Huang H
中科院分区：
文献类型：
--
作者：
Li K;Li R;Ni Y;Sun P;Liu Y;Zhang D;Huang H
背景精子选择对于通过辅助生殖技术（ART）受孕的后代的健康至关重要。为了获得更好的精子质量和避免潜在的遗传疾病，已经开发了各种用于体外受精和卵胞浆内单精子注射的精子制备方法。然而，目前的精子处理和选择技术绕过了体内受精过程中发生的自然选择。本研究的目的是提出一种新的距离孕酮结合的选择方法与原始设备的基础上，人类女性生殖道，并报告其有效性的基础上，精子的前向运动，以及chemotaxis.MethodsA新的装置与长距离的通道，模仿女性人类生殖系统的设计和制造。该即用型装置是使用孕酮梯度和人管液体培养基开发的。精子经Percoll梯度离心分离后，在37 °C、5%CO2的条件下在装置中游动150 min。使用Diff-Quik染色评估所选精子的正常形态。染色质扩散试验评估精子的DNA片段和凋亡进行了评估，使用膜联蛋白V-异硫氰酸荧光素/碘化丙啶荧光staining.ResultsOur距离孕酮结合精子选择方法成功地建立。精子经筛选后，形态正常的精子比例增加（选择前与选择后，11.2 ± 1.3%与40.3 ± 6.6%，P = 0.000），DNA片段化的精子百分比降低（15.4 ± 4.0%vs.6.8 ± 3.3%，P = 0.001），凋亡精子百分率无明显变化。该方法将有利于临床ART实践，因为它可以降低精子相关的遗传风险。
BackgroundSperm selection is essential for the health of offspring conceived via assistive reproductive technology (ART). Various methods of sperm preparation for in vitro fertilization and intracytoplasmic sperm injection have been developed to acquire sperm with better quality and to avoid potential genetic disorders. However, current sperm processing and selection techniques bypass the natural selection that occurs during fertilization in vivo. The aim of this study was to present a novel distance-progesterone-combined selection approach with an original device based on the human female reproductive tract, and to report on its effectiveness based on sperm progressive motility, as well as chemotaxis.MethodsA novel device with long distance channels which mimicked the female human reproductive system was designed and fabricated. This ready-to-be-used device was developed using a progesterone gradient and human tube fluid media. Sperm swam for 150 min in the device under conditions of 37 °C air temperature with 5% CO2after separation from seminal plasma via discontinuous Percoll gradient treatment. The selected sperm were assessed for normal morphology using Diff-Quik staining. A chromatin diffusion assay assessed sperm for DNA fragments and apoptosis was assessed using annexin V-fluorescein isothiocyanate/propidium iodide fluorescent staining.ResultsOur distance-progesterone-combined sperm selection method was successfully established. After sperm were selected, the percentage of sperm with normal morphology increased (before vs. after selection, 11.2 ± 1.3% vs. 40.3 ± 6.6%, P = 0.000), the percentage of sperm with DNA fragmentation decreased (before vs. after selection, 15.4 ± 4.0% vs. 6.8 ± 3.3%, P = 0.001), and the percentage of sperm with apoptosis did not change significantly.ConclusionsOur newly-developed method is capable of successfully selecting sperm of high quality. The method will be benefit clinical ART practice as it can reduce sperm-related genetic risks.
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影响因子：
2.9
作者：
Perez-Cerezales S;Boryshpolets S;Eisenbach M
通讯作者：
Eisenbach M
影响因子：
4
作者：
Glander, HJ;Schaller, J
通讯作者：
Schaller, J
影响因子：
2.4
作者：
Tavalaee, M.;Deemeh, M. R.;Nasr-Esfahani, M. H.
通讯作者：
Nasr-Esfahani, M. H.
影响因子：
3.7
作者：
Teves ME;Guidobaldi HA;Uñates DR;Sanchez R;Miska W;Publicover SJ;Morales Garcia AA;Giojalas LC
通讯作者：
Giojalas LC
影响因子：
13.3
作者：
Coutton, Charles;Escoffier, Jessica;Ray, Pierre F.
通讯作者：
Ray, Pierre F.