Phytochrome control of in vitro transcription of specific genes in isolated nuclei from barley (Hordeum vulgare).
Phytochrome control of in vitro transcription of specific genes in isolated nuclei from barley (Hordeum vulgare).
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光敏色素控制大麦（Hordeum vulgare）分离细胞核中特定基因的体外转录。
DOI：
10.1111/j.1432-1033.1985.tb08729.x
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Klaus Apel
中科院分区：
文献类型：
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作者：
E. Mösinger;Alfred Batschauer;Eberhard Schäfer;Klaus Apel
四个不同的基因在不同的光制度下生长的大麦芽的转录率进行了定量监测核RNA转录本使用基因特异性杂交探针。用[α-32 P]UTP脉冲标记分离的细胞核，测量捕光叶绿素a/B蛋白（LHCP）mRNA、NADPH：原叶绿素氧化还原酶mRNA、B1大麦醇溶蛋白mRNA和26-S rRNA合成的相对速率。用红光脉冲照射黑暗生长的植物，3小时内LHCP mRNA合成速率增加10倍，9小时内rRNA合成速率增加2倍以上。氧化还原酶mRNA合成的相对速率在红光脉冲后降低，在3-6 h后达到最小值。作为光敏色素参与LHCP的光诱导刺激和氧化还原酶转录物的抑制两种反应的直接证据，可以证明红色/远红可逆性。我们的结论是，光敏色素是既能够增加某些核基因的转录，并减少其他的转录。
The transcriptional rates of four different genes in shoots of barley grown under different light regimes were quantified by monitoring nuclear RNA transcripts using gene-specific hybridization probes. Isolated nuclei were pulse-labelled with [alpha-32P]UTP and the relative rates of light-harvesting chlorophyll a/b protein (LHCP) mRNA, NADPH:protochlorophyllide oxidoreductase mRNA, B1 hordein mRNA, and 26-S rRNA synthesis were measured. Irradiation of dark-grown plants with a red light pulse increased the rate of LHCP mRNA synthesis tenfold within 3 h, and the rate of rRNA synthesis more than twofold within 9 h. The relative rate of synthesis of the oxidoreductase mRNA decreased following a red light pulse reaching a minimum after 3-6 h. As a direct proof of phytochrome involvement in the light-induced stimulation of LHCP and the repression of the oxidoreductase transcripts for both responses, red/far-red reversibility could be demonstrated. We conclude that phytochrome is able both to increase the transcription of certain nuclear genes and decrease the transcription of others.
DOI：
--
发表时间：
1979-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Blackburn
通讯作者：
P. Blackburn