5-methylcytosine in RNA: detection, enzymatic formation and biological functions.
5-methylcytosine in RNA: detection, enzymatic formation and biological functions.
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DOI：
10.1093/nar/gkp1117
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发表时间：
2010-03
影响因子：
14.9
通讯作者：
Helm M
中科院分区：
文献类型：
--
作者：
Motorin Y;Lyko F;Helm M
核碱基修饰5-甲基胞嘧啶（m5 C）广泛存在于DNA和不同的细胞RNA中。在过去的几十年中，DNA m5 C-甲基化的功能和酶机制被广泛研究。然而，相同的修饰的核碱基在RNA中的位置、形成机制和细胞功能仍有待阐明。最近开发的亚硫酸氢盐测序方法，有效的m5 C定位在各种RNA分子中的核糖-m5 C在一个高度特权的位置，作为为数不多的RNA修饰，其检测是服从基于PCR的扩增和测序方法之一。该领域的其他进展还包括对各种生物体中几种特定RNA甲基转移酶的表征，以及发现DNA和RNA m5 C-甲基化之间新的和意想不到的联系。许多推定的RNA：m5 C-MTases现已被鉴定，并正在等待表征，包括鉴定其RNA底物及其相关的细胞功能。为了使这些最近令人兴奋的发展前景，本文综述了RNA甲基化的检测方法，相应的修饰酶的生物化学，酶学和分子生物学的有序概述，并讨论了这些酶的新兴生物学功能的前景。
The nucleobase modification 5-methylcytosine (m5C) is widespread both in DNA and different cellular RNAs. The functions and enzymatic mechanisms of DNA m5C-methylation were extensively studied during the last decades. However, the location, the mechanism of formation and the cellular function(s) of the same modified nucleobase in RNA still remain to be elucidated. The recent development of a bisulfite sequencing approach for efficient m5C localization in various RNA molecules puts ribo-m5C in a highly privileged position as one of the few RNA modifications whose detection is amenable to PCR-based amplification and sequencing methods. Additional progress in the field also includes the characterization of several specific RNA methyltransferase enzymes in various organisms, and the discovery of a new and unexpected link between DNA and RNA m5C-methylation. Numerous putative RNA:m5C-MTases have now been identified and are awaiting characterization, including the identification of their RNA substrates and their related cellular functions. In order to bring these recent exciting developments into perspective, this review provides an ordered overview of the detection methods for RNA methylation, of the biochemistry, enzymology and molecular biology of the corresponding modification enzymes, and discusses perspectives for the emerging biological functions of these enzymes.
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影响因子：
3.7
作者：
DUBIN, DT;STOLLAR, V;GUILD, GM
通讯作者：
GUILD, GM
影响因子：
15
作者：
Ali, M;Alam, R;Sasaki, S
通讯作者：
Sasaki, S
DOI：
10.1038/nsb0196-38
发表时间：
1996-01-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
Basti, MM;Stuart, JW;Agris, PF
通讯作者：
Agris, PF
影响因子：
2.9
作者：
CHEN, Y;SIERZPUTOWSKAGRACZ, H;AGRIS, PF
通讯作者：
AGRIS, PF
影响因子：
14.9
作者：
Bujnicki, JM;Feder, M;Redman, KL
通讯作者：
Redman, KL