Top-down proteomics reveals novel protein forms expressed in Methanosarcina acetivorans.
Top-down proteomics reveals novel protein forms expressed in Methanosarcina acetivorans.
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DOI：
10.1016/j.jasms.2009.05.014
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发表时间：
2009-09
影响因子：
3.2
通讯作者：
Kelleher NL
中科院分区：
文献类型：
--
作者：
Ferguson JT;Wenger CD;Metcalf WW;Kelleher NL
利用自动纳米喷雾和在线液相色谱质谱LC-MS策略，通过自上而下串联质谱（MS/MS）技术，从古菌中已知基因组最大的产甲烷菌Methanosarcina acetivorans中鉴定出99种蛋白质。由于使用了自上而下的MS/MS， 15个蛋白被检测到错误的起始位点，另外5个蛋白来自小的开放阅读框（sorf）。除了描述这些基因组注释中更常见的差异外，一个SORF是由一个罕见的起始密码子（AUA）作为该蛋白翻译的起始位点引起的。此外，30S核糖体蛋白（MA1259）的甲基化定位于Pro59-Val69，与大肠杆菌（rp S12）的同源物形成鲜明对比，已知其含有不寻常的β-硫甲基化天冬氨酸残基。
Using both automated nanospray and online liquid chromatography mass spectrometry LC-MS strategies, 99 proteins have been newly identified by top-down tandem mass spectrometry (MS/MS) in Methanosarcina acetivorans, the methanogen with the largest known genome [5.7 mega base pairs (Mb)] for an Archaeon. Because top-down MS/MS was used, 15 proteins were detected with mispredicted start sites along with an additional five from small open reading frames (SORFs). Beyond characterization of these more common discrepancies in genome annotation, one SORF resulted from a rare start codon (AUA) as the initiation site for translation of this protein. Also, a methylation on a 30S ribosomal protein (MA1259) was localized to Pro59–Val69, contrasting sharply from its homologue in Escherichia coli (rp S12) known to harbor an unusual β-thiomethylated aspartic acid residue.
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影响因子：
7
作者：
Patrie, SM;Ferguson, JT;Kelleher, NL
通讯作者：
Kelleher, NL
影响因子：
46.9
作者：
Meng, FY;Cargile, BJ;Kelleher, NL
通讯作者：
Kelleher, NL
影响因子：
7.4
作者：
Roth, Michael J.;Parks, Bryan A.;Kelleher, Neil L.
通讯作者：
Kelleher, Neil L.
DOI：
10.1093/dnares/3.4.225
发表时间：
1996-08-31
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Sazuka, T;Ohara, O
通讯作者：
Ohara, O
影响因子：
7.4
作者：
Collier, Timothy S.;Hawkridge, Adam M.;Muddiman, David C.
通讯作者：
Muddiman, David C.