Comparison of 18F PET and 99mTc SPECT imaging in phantoms and in tumored mice.
Comparison of 18F PET and 99mTc SPECT imaging in phantoms and in tumored mice.
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DOI：
10.1021/bc1001467
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发表时间：
2010-08-18
影响因子：
4.7
通讯作者：
Hnatowich, Donald J.
中科院分区：
文献类型：
--
作者：
Cheng, Dengfeng;Wang, Yi;Liu, Xinrong;Pretorius, P. Hendrik;Liang, Minmin;Rusckowski, Mary;Hnatowich, Donald J.
我们的目的是比较在Her2+肿瘤小鼠中使用99mTc和18F放射性标记的抗Her2纳米粒子的microSPECT和microPET的性能。首先使用模体比较相机性能；然后使用BioSCAN NanoSPECT/CT对注射99mTC-纳米颗粒的肿瘤小鼠进行成像，而另一只肿瘤小鼠接受相同的纳米颗粒，现在用18F标记，并在飞利浦Mosaic HP PET相机上成像。通过MAG3络合，以18F为中间体，用~(99m)Tc通过MAG3对纳米粒子进行放射性标记。Phantom成像显示，SPECT相机的分辨率明显优越，但即使使用4个头和多个针孔准直器，检测灵敏度也降低了15倍。与18F的人工共价连接相比，99mTc络合标记纳米粒子更容易、更安全。这两种相机都能在很大范围内提供准确的放射性定量。综上所述，当在99mTc和18F之间做出选择时，与18F的共价连接相比，99mTc的一个优势在于通过手动或其他方式实现的螯合作用，但对于这些小型动物相机，这种选择也会导致以较低的灵敏度换取较高的分辨率。
Our objective was to compare the performance of a microSPECT with that of a microPET in a Her2+ tumored mice using an anti-Her2 nanoparticle radiolabeled with 99mTc and 18F. Camera performance was first compared using phantoms; then a tumored mouse administered the 99mTc-nanoparticle was imaged on a Bioscan NanoSPECT/CT while another tumored mouse received the identical nanoparticle, labeled now with 18F, and was imaged on a Philips Mosaic HP PET camera. The nanoparticle was radiolabeled with 99mTc via MAG3 chelation and with 18F via SFB as intermediate. Phantom imaging showed that the resolution of the SPECT camera was clearly superior but even with 4 heads and multipinhole collimators, detection sensitivity was 15 fold lower. Radiolabeling of the nanoparticle by chelation with 99mTc was considerably easier and safer than manual covalent attachment of 18F. Both cameras provided accurate quantitation of radioactivity over a broad range. In conclusion, when deciding between 99mTc vs. 18F, an advantage rests with chelation of 99mTc over covalent attachment of 18F, achieved manually or otherwise, but with these small animal cameras, this choice also results in trading lower sensitivity for higher resolution.
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影响因子：
14.8
作者：
Wang, Yi;Liu, Xinrong;Hnatowich, Donald J.
通讯作者：
Hnatowich, Donald J.
DOI：
10.1007/s00259-008-0989-5
发表时间：
2009-03-01
影响因子：
9.1
作者：
Eshuis, S. A.;Jager, P. L.;Leenders, K. L.
通讯作者：
Leenders, K. L.
影响因子：
9.3
作者：
Moroz, Maxim A.;Serganova, Inna;Blasberg, Ronald G.
通讯作者：
Blasberg, Ronald G.
影响因子：
1.9
作者：
Cheng, Dengfeng;Yin, Duanzhi;Wang, Yongxian
通讯作者：
Wang, Yongxian
影响因子：
4.7
作者：
Wang, Yi;Nakamura, Kayoko;Hnatowich, Donald J.
通讯作者：
Hnatowich, Donald J.