Interactions of engineered nanomaterials in physiological media and implications for in vitro dosimetry.
Interactions of engineered nanomaterials in physiological media and implications for in vitro dosimetry.
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DOI：
10.3109/17435390.2012.666576
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发表时间：
2013-06
期刊：
影响因子：
5
通讯作者：
Demokritou P
中科院分区：
文献类型：
--
作者：
Cohen J;Deloid G;Pyrgiotakis G;Demokritou P
体外毒性测定是筛选进入消费者市场的越来越多的工程纳米材料（ENM）的有效和廉价的工具。然而，体外研究产生的数据往往在不同的研究和体内数据之间存在很大差异。在某种程度上，这些差异可能是由于分散方案缺乏标准化和颗粒-介质相互作用的表征不足，这可能影响颗粒动力学和递送至细胞的剂量。在这项研究中，提出了一种新的方法，用于制备单分散，稳定的液体悬浮液，再加上一个数值模型，估计交付的剂量值。对于可用于将施用剂量转换为递送剂量的每种介质- ENM系统，推导出经验推导的材料特异性和介质特异性函数。研究了ENM与各种生理介质的相互作用，并通过使用THP-1巨噬细胞的体外细胞毒性测定证明了这种方法的重要性。
In vitro toxicity assays are efficient and inexpensive tools for screening the increasing number of engineered nanomaterials (ENMs) entering the consumer market. However, the data produced by in vitro studies often vary substantially among different studies and from in-vivo data. In part these discrepancies may be attributable to lack of standardization in dispersion protocols and inadequate characterization of particle-media interactions which may affect the particle kinetics and the dose delivered to cells. In this study, a novel approach for preparation of monodisperse, stabilized liquid suspensions was presented and coupled with a numerical model which estimates delivered dose values. Empirically derived material- and media-specific functions were derived for each media- ENM system that can be used to convert administered to delivered doses. The interactions of ENMs with a variety of physiologic media were investigated and the importance of this approach was demonstrated by in vitro cytotoxicity assays using THP-1 macrophages.
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影响因子：
10
作者：
Hinderliter PM;Minard KR;Orr G;Chrisler WB;Thrall BD;Pounds JG;Teeguarden JG
通讯作者：
Teeguarden JG
影响因子：
3.7
作者：
DeLoid, Glen M.;Sulahian, Timothy H.;Kobzik, Lester
通讯作者：
Kobzik, Lester
DOI：
10.1080/10937404.2010.483176
发表时间：
2010-02
期刊：
Journal of toxicology and environmental health. Part B, Critical reviews
影响因子：
--
作者：
Krewski D;Acosta D Jr;Andersen M;Anderson H;Bailar JC 3rd;Boekelheide K;Brent R;Charnley G;Cheung VG;Green S Jr;Kelsey KT;Kerkvliet NI;Li AA;McCray L;Meyer O;Patterson RD;Pennie W;Scala RA;Solomon GM;Stephens M;Yager J;Zeise L
通讯作者：
Zeise L
影响因子：
3.8
作者：
Murdock, Richard C.;Braydich-Stolle, Laura;Hussain, Saber M.
通讯作者：
Hussain, Saber M.
影响因子：
10.4
作者：
OBERDORSTER, G;FERIN, J;LEHNERT, BE
通讯作者：
LEHNERT, BE