An atractylodes macrocephala koidz extract alleviates hyperandrogenism of polycystic ovarian syndrome
An atractylodes macrocephala koidz extract alleviates hyperandrogenism of polycystic ovarian syndrome
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白术提取物缓解多囊卵巢综合征的高雄激素血症
DOI：
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发表时间：
2016
影响因子：
0.1
通讯作者：
Hardiman Paul J.
中科院分区：
文献类型：
--
作者：
Zhou Jue;Qu Fan;Barry John A.;Pan Jie-Xue;Wang Fang-Fang;Fu Zhen-Zhen;Duez Pierre;Hardiman Paul J.
背景：白术是亚洲大多数国家普遍采用的补益中药，临床上多以白术为主，治疗多囊卵巢综合征(PCOS)。目的：探讨黄芪极地提取物(EAMK)对丙酸睾丸酮诱导的多囊卵巢综合征(PCOS)模型大鼠的影响。材料和方法：60只大鼠随机分为5组，每组12只：健康对照组、未治疗组、治疗组(EAMK高、中、低剂量组)。出生后第9天单次注射丙酸睾丸酮诱导PCOS。用双抗体夹心酶联免疫吸附测定法测定血浆总睾酮(TT)、性激素结合球蛋白(SHBG)、雄烯二酮、黄体生成素(LH)、卵泡刺激素(FSH)、抗利尿激素(AMH)水平。用实时定量聚合酶链式反应和免疫组织化学方法检测FSH受体(FSHR)和水通道蛋白-9(AQP-9)在大鼠卵巢中的表达。我们还检测了在没有和存在双氢睾酮(DHT)的情况下，FSHR和AQP-9在对照组和EAMK处理组的人卵巢颗粒样KGN细胞中的表达。结果：EMAK能明显改善PCOS大鼠的动情周期，降低血浆TT、FAI和雄烯二酮水平，并呈剂量依赖关系(P<；0.001)。在PCOS大鼠(P<；0.001)和双羟色胺处理的KGN细胞(P<；0.001)中，EAMK处理组均显著降低FSHR的表达水平，增加AQP-9的表达，且呈剂量依赖关系。结论：EAMK可减轻PCOS大鼠高雄激素血症，调节卵巢表达。
Background: Atractylodes macrocephala Koidz (AMK) is a tonic herb widely prescribed in most Asian countries, which has been clinically used as the dominant herb in most of the Chinese medicinal formula of treating polycystic ovarian syndrome (PCOS). Objective: To explore the effects of a polar extract of AMK (EAMK) in a hyperandrogenic rat model of PCOS induced by testosterone propionate. Material and methods: Sixty rats were randomly allocated to five groups of 12: healthy controls; untreated PCOS; three groups of treated PCOS (high, medium and low-dose EAMK). PCOS was induced with a single injection of testosterone propionate on the ninth day after birth. The plasma levels of total testosterone (TT), sex hormone binding globulin (SHBG), androstenedione, luteinizing hormone (LH), follicle stimulating hormone (FSH), antimüllerian hormone (AMH) were measured by enzyme-linked immunosorbent assays. The expression of FSH receptors (FSHR) and aquaporin-9 (AQP-9) in the ovaries of the rats was measured by real-time quantitative PCR and immunohistochemistry. We also measured the expression of FSHR and AQP-9 in human ovarian granulosa-like KGN cells, control and EAMK-treated, in the absence and presence of dihydrotestosterone (DHT). Results: EMAK significantly improved the estrous cycles, decreased the plasma levels of TT, FAI and androstenedione of the PCOS rats in a dose-dependent manner (P < 0.01) and the EAMK groups displayed significantly higher plasma FSH levels and lower LH/FSH and AMH levels than the PCOS control group (P < 0.001). In a dose-dependent manner, the EAMK treatments significantly decreased the FSHR expression levels and increased AQP-9 expression both in the ovaries of PCOS rats (P < 0.001) and in the KGN cells treated with DHT (P < 0.001). Conclusions: EAMK alleviates the PCOS rats’ hyperandrogenism and regulates the ovarian expression
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DOI：
10.4314/ajtcam.v8i4.10
发表时间：
2011
期刊：
African journal of traditional, complementary, and alternative medicines : AJTCAM
影响因子：
--
作者：
Jue Zhou;F. Qu;Yongping Yu
通讯作者：
Jue Zhou;F. Qu;Yongping Yu
影响因子：
6.1
作者：
Qu, Fan;Wang, Fang-Fang;Huang, He-Feng
通讯作者：
Huang, He-Feng
影响因子：
6.1
作者：
Tang, T;Glanville, J;Balen, AH
通讯作者：
Balen, AH
影响因子：
2.6
作者：
Wang, Jianbo;Tanji, Nozomu;Yokoyama, Masayoshi
通讯作者：
Yokoyama, Masayoshi
影响因子：
3.6
作者：
Pastor-Soler, L;Isnard-Bagnis, C;Breton, S
通讯作者：
Breton, S