Repurposing ribosomes for synthetic biology.
Repurposing ribosomes for synthetic biology.
复制标题
DOI：
10.1016/j.cbpa.2017.07.012
复制
发表时间：
2017-10
影响因子：
7.8
通讯作者：
Jewett MC
中科院分区：
文献类型：
--
作者：
Liu Y;Kim DS;Jewett MC
翻译系统是细胞的蛋白质生物合成工厂，将成百上千个较小的氨基酸单位缝合成蛋白质，这些蛋白质是生命系统的结构、功能和调节所必需的。该系统包括聚合所需的核糖体及其相关因子，其非凡的合成能力推动了广泛的努力，以利用它来满足能源、材料和医药等不同领域的社会需求。一个例子是重组蛋白的生产，它通过合成胰岛素等生物药物影响了患者的生活。然而，在自然界中，只有有限的单体被利用，从而导致生物聚合物(即蛋白质)的有限的集合。扩展自然界的核糖体单体库可能会产生具有不同遗传编码化学的新类别的酶、治疗、材料和化学物质。在这里，我们讨论了在体内和体外工程核糖体的最新进展。这些根本性和技术性的突破为生物技术和合成生物学的高级应用打开了大门。
The translation system is the cell’s factory for protein biosynthesis, stitching together hundreds to thousands of smaller units of amino acids into proteins, which are required for the structure, function, and regulation of living systems. The extraordinary synthetic capability of this system, which includes the ribosome and its associated factors required for polymerization, has driven extensive efforts to harness it for societal needs in areas as diverse as energy, materials, and medicine. An example is recombinant protein production that has impacted the lives of patients through the synthesis of biopharmaceuticals such as insulin. In nature, however, only limited sets of monomers are utilized, thereby resulting in limited sets of biopolymers (i.e., proteins). Expanding nature’s repertoire of ribosomal monomers could yield new classes of enzymes, therapeutics, materials, and chemicals with diverse genetically encoded chemistry. Here, we discuss recent progress towards engineering ribosomes both in vivo and in vitro. These fundamental and technical breakthroughs open doors for advanced applications in biotechnology and synthetic biology.
登录
查看更多内容
影响因子：
14.9
作者：
Chubiz LM;Rao CV
通讯作者：
Rao CV
影响因子：
4.5
作者：
Goto, Yuki;Murakami, Hiroshi;Suga, Hiroaki
通讯作者：
Suga, Hiroaki
影响因子：
2.9
作者：
Dedkova, Larisa M.;Fahmi, Nour Eddine;Hecht, Sidney M.
通讯作者：
Hecht, Sidney M.
大分子拥挤和减少体外核糖体构建条件的影响。
影响因子：
14.9
作者：
Fritz BR;Jamil OK;Jewett MC
通讯作者：
Jewett MC
影响因子：
7.8
作者：
Des Soye, Benjamin J.;Patel, Jaymin R.;Isaacs, Farren J.;Jewett, Michael C.
通讯作者：
Jewett, Michael C.