Constitutive activity of inwardly rectifying K+ channel at physiological [Ca]i is mediated by Ca2+/CaMK II pathway in opossum kidney proximal tubule cells.
Constitutive activity of inwardly rectifying K+ channel at physiological [Ca]i is mediated by Ca2+/CaMK II pathway in opossum kidney proximal tubule cells.
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负鼠肾近曲小管细胞中生理学 [Ca]i 的内向整流 K 通道的组成活性是由 Ca2 /CaMK II 途径介导的。
DOI：
10.2170/physiolsci.rp014507
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
T. Kubota
中科院分区：
文献类型：
--
作者：
Y. Mori;H. Yoshida;M. Miyamoto;Y. Sohma;T. Kubota
采用膜片钳技术，研究了钙/钙调蛋白激酶II（CaMK II）介导的磷酸化过程对负鼠肾近曲小管细胞（OKPCs）内向电导为90 pS的K+通道的作用。通过使用荧光染料Fura 2测量细胞内Ca 2+浓度（[Ca]i）。获得了以下结果：（i）在细胞附着的贴片中，通过灌流低Ca 2+（10（-8）M）、La 3+（100 μ M）或EGTA/AM（100 μ M）引起的[Ca]i降低抑制了通道活性浴溶液中。CaMK II抑制剂KN-62（10 μ M）或KN-93（5 μ M）的应用也抑制了通道活性。(ii)用制霉菌素穿孔贴片测量的膜电位显著降低，这是由于用含EGTA或La（3+）的溶液灌注引起的[Ca]i下降。此外，KN-62（10 μ M）或KN-93（5 μ M）的应用浴显着降低膜电位。(iii)在由内向外的贴片中，在浴中施加10（-7）M Ca 2+的CaMK II（300 pM）显著刺激通道活性。此外，KN-62（10 μ M）的应用程序的浴显着降低通道活性。我们的研究结果表明，在生理[Ca]i的内向整流K+通道的组成性活动介导的Ca 2 +/CaMK Ⅱ途径在OKPCs。
Using patch-clamp technique, we studied the role of the Ca2+/calmodulin kinase II (CaMK II)-mediated phosphorylation process on the K+ channel with an inward conductance of 90 pS in opossum kidney proximal tubule cells (OKPCs). The intracellular Ca2+ concentration ([Ca]i) was measured by use of the fluorescent dye fura 2. The following results were obtained: (i) In cell-attached patches, the channel activity was inhibited by a decrease in [Ca]i induced by perfusion with low Ca2+ (10(-8) M), La3+ (100 microM), or EGTA/AM (100 microM) contained in the bath solution. The application of KN-62 (10 microM) or KN-93 (5 microM), inhibitors of CaMK II, also inhibited the channel activity. (ii) The membrane potential measured with nystatin-perforated patches was significantly decreased by the fall in [Ca]i induced by the perfusion with EGTA- or La(3+)-containing solution. Also, the application of KN-62 (10 microM) or KN-93 (5 microM) to the bath significantly decreased the membrane potential. (iii) In inside-out patches, the channel activity was significantly stimulated by the application of CaMK II (300 pM) at 10(-7) M Ca2+ in the bath. Furthermore, the application of KN-62 (10 microM) to the bath significantly decreased the channel activity. Our findings show that the constitutive activity of inwardly rectifying K+ channel at physiological [Ca]i is mediated by the Ca2+/CaMK II pathway in OKPCs.
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DOI：
10.1007/978-1-4899-6778-7_6
发表时间：
1987
期刊：
International journal of neurology
影响因子：
--
作者：
T. Teyler
通讯作者：
T. Teyler
DOI：
10.1152/ajprenal.2000.279.3.f518
发表时间：
2000-09
期刊：
American journal of physiology. Renal physiology
影响因子：
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作者：
Tong Wang;Fiona M. Inglis;Robert G. Kalb
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Tong Wang;Fiona M. Inglis;Robert G. Kalb
Ca2 /CaMK II 在 Ca(2 ) 诱导的大鼠 CCD 主细胞 K 通道抑制中的作用。
DOI：
10.1152/ajprenal.1995.268.2.f211
发表时间：
1995
期刊：
The American journal of physiology
影响因子：
--
作者：
Kubokawa,M;Wang,W;McNicholas,CM;Giebisch,G
通讯作者：
Giebisch,G
DOI：
10.1152/ajprenal.00376.2005
发表时间：
2006-05-01
影响因子：
4.2
作者：
Goel, M;Sinkins, WG;Schilling, WP
通讯作者：
Schilling, WP
哺乳动物肾小管的电生理学：细胞内微电极研究的推论。
影响因子：
18.2
作者：
Koeppen,BM;Giebisch,G;Biagi,BA
通讯作者：
Biagi,BA