Poly-Epsilon-Lysine Hydrogels with Dynamic Crosslinking Facilitates Cell Proliferation.
Poly-Epsilon-Lysine Hydrogels with Dynamic Crosslinking Facilitates Cell Proliferation.
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DOI：
10.3390/ma13173851
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发表时间：
2020-09-01
期刊：
影响因子：
--
通讯作者：
Bradley M
中科院分区：
文献类型：
--
作者：
Lopez Mora N;Owens M;Schmidt S;Silva AF;Bradley M
细胞外基质 (ECM) 是一个三维网络，其中细胞附着、增殖和分化等基本细胞过程由其固有的生物和结构线索驱动发生。水凝胶已被用作生物材料，因为它们具有许多控制细胞过程的 ECM 特性。然而，水凝胶中常见的永久交联无法概括天然 ECM 的动态性质。这不仅阻碍自然细胞迁移，而且还必须限制细胞扩张和生长。此外，人们对使用新型生物聚合物来制造可用于生物医学应用的仿生材料越来越感兴趣。在这里，我们报告了天然聚合物聚-ε-赖氨酸通过可逆亚胺键形成形成动态水凝胶，并通过精氨酸-甘氨酸-天冬氨酸（RGD）掺入促进细胞附着。总之，具有低聚ε-赖氨酸含量的动态水凝胶的机械性能和细胞行为表明良好的细胞活力和高代谢活性。
The extracellular matrix (ECM) is a three-dimensional network within which fundamental cell processes such as cell attachment, proliferation, and differentiation occur driven by its inherent biological and structural cues. Hydrogels have been used as biomaterials as they possess many of the ECM characteristics that control cellular processes. However, the permanent crosslinking often found in hydrogels fails to recapitulate the dynamic nature of the natural ECM. This not only hinders natural cellular migration but must also limit cellular expansion and growth. Moreover, there is an increased interest in the use of new biopolymers to create biomimetic materials that can be used for biomedical applications. Here we report on the natural polymer poly-ε-lysine in forming dynamic hydrogels via reversible imine bond formation, with cell attachment promoted by arginine-glycine-aspartic acid (RGD) incorporation. Together, the mechanical properties and cell behavior of the dynamic hydrogels with low poly-ε-lysine quantities indicated good cell viability and high metabolic activity.
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影响因子：
10.8
作者：
Martinez-Sanz, Elena;Ossipov, Dmitri A.;Varghese, Oommen P.
通讯作者：
Varghese, Oommen P.
DOI：
10.1007/s10856-019-6303-1
发表时间：
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影响因子：
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