Discovery of a novel swine enteric alphacoronavirus (SeACoV) in southern China.
Discovery of a novel swine enteric alphacoronavirus (SeACoV) in southern China.
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在中国南方发现一种新型猪肠道α冠状病毒（SeACoV）
DOI：
10.1016/j.vetmic.2017.09.020
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发表时间：
2017-11
影响因子：
3.3
通讯作者：
Huang YW
中科院分区：
文献类型：
--
作者：
Pan Y;Tian X;Qin P;Wang B;Zhao P;Yang YL;Wang L;Wang D;Song Y;Zhang X;Huang YW
一种新型猪肠道甲型冠状病毒（暂命名为SeACoV）从中国南方腹泻仔猪体内分离得到。
SeACoV在抗原性上可能与猪流行性腹泻病毒（PEDV）、猪传染性胃肠炎病毒（TGEV）及猪德尔塔冠状病毒（PDCoV）不同。
基因组及系统发育分析表明，SeACoV可能起源于蝙蝠冠状病毒HKU2。
与HKU2相比，SeACoV刺突糖蛋白的极端氨基末端结构域具有极高的变异性。
实验感染研究显示，SeACoV对新生仔猪具有感染性和致病性。
自2017年2月起，中国南方一家猪场记录到新生仔猪发生腹泻疫情，但未检测到猪传染性胃肠炎病毒（TGEV）、猪流行性腹泻病毒（PEDV）和猪德尔塔冠状病毒（PDCoV）。通过在细胞培养中对病原体进行分离和增殖，发现了一种新型猪肠道甲型冠状病毒（暂命名为SeACoV），该病毒与十年前在同一地区鉴定出的蝙蝠冠状病毒HKU2相关。使用从同一猪场采集的阳性母猪血清，在感染SeACoV的Vero细胞中检测到特异性荧光信号，但使用TGEV、PEDV或PDCoV特异性抗体时则未检测到。电子显微镜观察表明，该病毒粒子带有表面突起，直径为100 - 120纳米。SeACoV的全基因组测序及分析表明，SeACoV刺突（S）糖蛋白的极端氨基末端结构域在结构上与甲型冠状病毒NL63的结构域0相似，而S蛋白的其余部分在结构上与乙型冠状病毒的B至D结构域相似。与HKU2相比，与肠道嗜性相关的SeACoV - S结构域0具有极高的变异性，存在75个氨基酸（aa）替换和2个氨基酸插入，这可能是宿主范围扩大或跨物种传播的原因。将分离出的病毒经口服接种给3日龄新生仔猪后具有感染性，可导致仔猪出现腹泻的临床症状并从粪便中排出病毒。这些结果证实，它是一种新型猪肠道冠状病毒，代表着第五种猪冠状病毒。
A novel swine enteric alphacoronavirus (tentatively named SeACoV) was isolated from diarrheic piglets in southern China. SeACoV is likely antigenetically distinct from PEDV, TGEV and PDCoV. Genomic and phylogenetic analysis showed that SeACoV might have originated from the bat coronavirus HKU2. The extreme amino-terminal domain of SeACoV spike glycoprotein had an extremely high variability compared to that of HKU2. Experimental infection study showed that SeACoV is infectious and pathogenic in newborn piglets. Outbreaks of diarrhea in newborn piglets without detection of transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV) and porcine deltacoronavirus (PDCoV), have been recorded in a pig farm in southern China since February 2017. Isolation and propagation of the pathogen in cell culture resulted in discovery of a novel swine enteric alphacoronavirus (tentatively named SeACoV) related to the bat coronavirus HKU2 identified in the same region a decade ago. Specific fluorescence signal was detected in Vero cells infected with SeACoV by using a positive sow serum collected in the same farm, but not by using TGEV-, PEDV- or PDCoV-specific antibody. Electron microscopy observation demonstrated that the virus particle with surface projections was 100–120 nm in diameter. Complete genomic sequencing and analyses of SeACoV indicated that the extreme amino-terminal domain of the SeACoV spike (S) glycoprotein structurally similar to the domain 0 of the alphacoronavirus NL63, whereas the rest part of S structurally resembles domains B to D of the betacoronavirus. The SeACoV-S domain 0 associated with enteric tropism had an extremely high variability, harboring 75-amino-acid (aa) substitutions and a 2-aa insertion, compared to that of HKU2, which is likely responsible for the extended host range or cross-species transmission. The isolated virus was infectious in pigs when inoculated orally into 3-day-old newborn piglets, leading to clinical signs of diarrhea and fecal virus shedding. These results confirmed that it is a novel swine enteric coronavirus representing the fifth porcine coronavirus.
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影响因子：
11.8
作者：
Gong L;Li J;Zhou Q;Xu Z;Chen L;Zhang Y;Xue C;Wen Z;Cao Y
通讯作者：
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影响因子：
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作者：
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影响因子：
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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