Enhancement of pullulanase production from recombinant Bacillus subtilis by optimization of feeding strategy and fermentation conditions
Enhancement of pullulanase production from recombinant Bacillus subtilis by optimization of feeding strategy and fermentation conditions
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通过优化补料策略和发酵条件提高重组枯草芽孢杆菌的支链淀粉酶产量
DOI：
10.1186/s13568-020-0948-5
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发表时间：
2020-01
期刊：
影响因子：
3.7
通讯作者：
Yan Xu
中科院分区：
文献类型：
--
作者：
Yu Zhang;Yao Nie;Xia Zhou;Jiahua Bi;Yan Xu
Pullulanase is an important starch-debranching enzyme mostly used in starch processing-related food industries. However, the levels of pullulanase produced from recombinant Bacillus subtilis, a Generally Recognized as Safe (GRAS) host, are generally limited. To enhance the activity of pullulanase, batch fermentation and fed-batch fermentation were systematically investigated. The overall purpose is to improve the fermentation yield by optimizing the feeding strategy in the fermentation process, thereby increasing the enzyme activity of pullulanase. Therefore, in this study, the feeding methods, the feeding ingredients, the feeding concentration, and pH values were studied in detail. The optimized fermentation conditions for pullulanase production from recombinant B. subtilis were determined as following: inoculum volume 7%, pH 6.5, the dissolved oxygen level 30%, and constant-rate feeding of 100 mL glucose solution (400 g L−1) in late logarithmic growth. The OD600 of recombinant B. subtilis and enzyme activity were 84.54 and 102.75 U mL−1, which were respectively 141% and 144% higher than that before optimization. These findings provided a prerequisite for further amplification of the fermentation system to obtain higher enzyme activity.
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影响因子：
11.2
作者：
R. Singh;G. Saini;J. Kennedy
通讯作者：
R. Singh;G. Saini;J. Kennedy
影响因子：
16.6
作者：
Wang, Xin;Xia, Kang;Tang, Chao
通讯作者：
Tang, Chao
通过增强子调节改善重组枯草芽孢杆菌中长野芽孢杆菌支链淀粉酶的诱导表达。
影响因子：
1.6
作者：
Deng, Yi;Nie, Yao;Xu, Yan
通讯作者：
Xu, Yan
影响因子：
2.9
作者：
H. Prentice;B. Ehrenfels;W. Sisk
通讯作者：
H. Prentice;B. Ehrenfels;W. Sisk
影响因子：
3.2
作者：
Long Liu;G. Du;Jian Chen;Miao Wang;Jun Sun
通讯作者：
Long Liu;G. Du;Jian Chen;Miao Wang;Jun Sun