Improved urethral fluorescence during low rectal surgery: a new dye and a new method.
Improved urethral fluorescence during low rectal surgery: a new dye and a new method.
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DOI：
10.1007/s10151-018-1757-6
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发表时间：
2018-03
影响因子：
3.3
通讯作者：
Hompes R
中科院分区：
文献类型：
--
作者：
Barnes TG;Volpi D;Cunningham C;Vojnovic B;Hompes R
本研究的目的是通过使用两种不同的方法来证明手术期间尿道的突出：新的近红外荧光团IRDye 800 BK和与硅胶混合的吲哚菁绿色（ICG）。来自牛津大学解剖学系的男性尸体被用来在近红外荧光激发期间可视化尿道。为了评估IRDye 800 BK，在用与Instillagel混合的IRDye 800 BK溶液直接浸润尿道后使用会阴切口。在模拟经肛门全直肠系膜切除术中，故意暴露尿道时，对ICG硅胶进行了评估。ICG之前与乙醇和硅胶混合，并放置在Foley导管中。使用内部制造的荧光启用腹腔镜系统可视化荧光。IRDye 800 BK在荧光下在估计的2 cm组织深度处表现出优异的穿透性和尿道可视化。ICG-硅胶导管显示出优异的荧光，在其移除后在尿道中没有留下任何残留溶液。新描述的ICG-硅胶方法为荧光引导手术领域的新技术开辟了可能性。IRDye 800 BK是ICG在使用荧光成像可视化尿道方面的一种有前途的替代品。其更大的穿透深度可以允许在手术期间更早地检测尿道，并且更快地防止错误的平面手术。本文的在线版本（10.1007/s10151-018-1757-6）包含补充材料，可供授权用户使用。
The aim of this study was to demonstrate highlighting of the urethra during surgery through the use of two different methods: a new near-infrared fluorophore IRDye800BK, and indocyanine green (ICG) mixed with silicone. Male cadavers from the department of anatomy at the University of Oxford were used to visualise the urethra during near-infrared fluorescence excitation. To assess IRDye800BK, a perineal incision was utilised after infiltrating the urethra directly with an IRDye800BK solution mixed with Instillagel. ICG-silicone was assessed when the urethra was purposely exposed as part of a simulated transanal total mesorectal dissection. ICG was previously mixed with ethanol and silicone and left to set in a Foley catheter. Fluorescence was visualised using an in-house manufactured fluorescence-enabled laparoscopic system. IRDye800BK demonstrated excellent penetration and visualisation of the urethra under fluorescence at an estimated tissue depth of 2 cm. An ICG-silicone catheter demonstrated excellent fluorescence without leaving any residual solution behind in the urethra after its removal. The newly described ICG-silicone method opens up the possibility of new technologies in this area of fluorescence guided surgery. IRDye800BK is a promising alternative to ICG in visualising the urethra using fluorescence imaging. Its greater depth of penetration may allow earlier detection of the urethra during surgery and prevent wrong plane surgery sooner. The online version of this article (10.1007/s10151-018-1757-6) contains supplementary material, which is available to authorized users.
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影响因子：
4.6
作者：
Geissler, Daniel;Linden, Stina;Hildebrandt, Niko
通讯作者：
Hildebrandt, Niko
影响因子：
3.3
作者：
Atallah, S.;Albert, M.;Monson, J. R. T.
通讯作者：
Monson, J. R. T.
影响因子：
3.4
作者：
Burke, J. P.;Martin-Perez, B.;Atallah, S.
通讯作者：
Atallah, S.
影响因子：
48
作者：
Schneider CA;Rasband WS;Eliceiri KW
通讯作者：
Eliceiri KW
影响因子：
3.4
作者：
Penna, M.;Whiteford, M.;Sylla, P.
通讯作者：
Sylla, P.