Functional correlates of positional and gender-specific renal asymmetry in Drosophila.
Functional correlates of positional and gender-specific renal asymmetry in Drosophila.
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DOI：
10.1371/journal.pone.0032577
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Dow JA
中科院分区：
文献类型：
--
作者：
Chintapalli VR;Terhzaz S;Wang J;Al Bratty M;Watson DG;Herzyk P;Davies SA;Dow JA
在人类和其他动物中，内部器官不对称地位于体腔中，这种身体计划的破坏对人类来说可能是致命的。建立内部不对称的机制目前是密集研究的主题；然而，内部不对称(大脑外)的功能意义在很大程度上还没有被探索。内部不对称在功能上意义重大，还是仅仅是将器官塞进腔内的一种权宜之计？像人类一样，果蝇表现出内部不对称，肠道被扔进老套的褶皱中。还有肾脏不对称，最右边的一对(马氏管)总是在前面分支，最左边的一对总是坐在体腔的后面。因此，我们比较了成年雄性果蝇和雌性果蝇肾小管前向(右侧)和后向(左侧)的转录本。尽管编码小管基本功能(运输、信号)的基因是统一表达的，但一些功能(如先天免疫)在侧重点上表现出位置或性别的差异；其他功能，如钙的处理或潜在有毒氨的产生，则分别保留在右侧或左侧的小管上。这些发现与体腔内每对小管的不同位置有关。众所周知的发育基因(如背脊、腊肠和双性)在成体小管中持续、有模式地表达，这意味着躯体组织在一生中保持左右和性别认同。性别不对称也被注意到，无论是在防御方面，还是在神经肽F和性肽受体的男性特异性表达上：NPF只在男性肾小管中增加钙。因此，体腔内小管的物理不对称性是直接适应的。现在，内部不对称性背后的详细机制已经开始被描绘出来，我们的工作邀请人们进行调查，不仅是对孤立的组织，而且是在它们独特的物理位置和环境的背景下。
In humans and other animals, the internal organs are positioned asymmetrically in the body cavity, and disruption of this body plan can be fatal in humans. The mechanisms by which internal asymmetry are established are presently the subject of intense study; however, the functional significance of internal asymmetry (outside the brain) is largely unexplored. Is internal asymmetry functionally significant, or merely an expedient way of packing organs into a cavity? Like humans, Drosophila shows internal asymmetry, with the gut thrown into stereotyped folds. There is also renal asymmetry, with the rightmost pair of renal (Malpighian) tubules always ramifying anteriorly, and the leftmost pair always sitting posteriorly in the body cavity. Accordingly, transcriptomes of anterior-directed (right-side) and posterior-directed (left-side) Malpighian (renal) tubules were compared in both adult male and female Drosophila. Although genes encoding the basic functions of the tubules (transport, signalling) were uniformly expressed, some functions (like innate immunity) showed positional or gender differences in emphasis; others, like calcium handling or the generation of potentially toxic ammonia, were reserved for just the right-side or left-side tubules, respectively. These findings correlated with the distinct locations of each tubule pair within the body cavity. Well known developmental genes (like dorsocross, dachshund and doublesex) showed continuing, patterned expression in adult tubules, implying that somatic tissues maintain both left-right and gender identities throughout life. Gender asymmetry was also noted, both in defence and in male-specific expression of receptors for neuropeptide F and sex-peptide: NPF elevated calcium only in male tubules. Accordingly, the physical asymmetry of the tubules in the body cavity is directly adaptive. Now that the detailed machinery underlying internal asymmetry is starting to be delineated, our work invites the investigation, not just of tissues in isolation, but in the context of their unique physical locations and milieux.
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影响因子：
19.6
作者：
Chen, Yung-Hsiang;Liu, Hsin-Ping;Chen, Wen-Chi
通讯作者：
Chen, Wen-Chi
影响因子：
5.5
作者：
Broderick, KE;MacPherson, MR;Davies, SA
通讯作者：
Davies, SA
DOI：
10.1196/annals.1327.001
发表时间：
2005-01-01
期刊：
TRENDS IN COMPARATIVE ENDOCRINOLOGY AND NEUROBIOLOGY
影响因子：
--
作者：
Coast, GM;Garside, CS
通讯作者：
Garside, CS
影响因子：
4.8
作者：
Drago, Ilaria;Giacomello, Marta;Pozzan, Tullio
通讯作者：
Pozzan, Tullio
影响因子：
3
作者：
Eden, Eran;Navon, Roy;Yakhini, Zohar
通讯作者：
Yakhini, Zohar