The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity.
The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity.
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钙渗透通道OSCA1.3调节植物的气孔免疫。
DOI：
10.1038/s41586-020-2702-1
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发表时间：
2020-09
期刊：
影响因子：
64.8
通讯作者：
Zipfel C
中科院分区：
文献类型：
--
作者：
Thor K;Jiang S;Michard E;George J;Scherzer S;Huang S;Dindas J;Derbyshire P;Leitão N;DeFalco TA;Köster P;Hunter K;Kimura S;Gronnier J;Stransfeld L;Kadota Y;Bücherl CA;Charpentier M;Wrzaczek M;MacLean D;Oldroyd GED;Menke FLH;Roelfsema MRG;Hedrich R;Feijó J;Zipfel C
对生物和非生物胁迫的感知通常会导致植物气孔关闭。钙离子 (Ca2+) 穿过质膜的快速流入在这种反应中发挥着重要作用，但所涉及的 Ca2+ 通道的身份仍然难以捉摸。在这里，我们报道拟南芥 Ca2+ 通透通道 OSCA1.3 在免疫信号传导过程中控制气孔关闭。 OSCA1.3 在感知到病原体相关分子模式 (PAMP) 后迅速磷酸化。生化和定量磷酸蛋白质组学分析表明，在用肽 PAMP flg22（源自细菌鞭毛蛋白）处理后几分钟内，免疫受体相关胞质激酶 BIK1 与 OSCA1.3 的 N 端胞质环相互作用并磷酸化。遗传和电生理学数据表明，OSCA1.3 可渗透 Ca2+，BIK1 介导的 N 末端磷酸化可增加该通道的活性。值得注意的是，OSCA1.3 及其被 BIK1 磷酸化对于免疫信号传导期间气孔关闭至关重要，并且 OSCA1.3 不会在感知脱落酸（一种与非生物胁迫相关的植物激素）时调节气孔关闭。因此，这项研究确定了植物 Ca2+ 通道及其在免疫信号传导过程中气孔关闭的激活机制，并表明 Ca2+ 流入机制响应不同胁迫的特异性。
Perception of biotic and abiotic stresses often leads to stomatal closure in plants. Rapid influx of calcium ions (Ca2+) across the plasma membrane has an important role in this response, but the identity of the Ca2+ channels involved has remained elusive. Here we report that the Arabidopsis thaliana Ca2+-permeable channel OSCA1.3 controls stomatal closure during immune signalling. OSCA1.3 is rapidly phosphorylated upon perception of pathogen-associated molecular patterns (PAMPs). Biochemical and quantitative phosphoproteomics analyses reveal that the immune receptor-associated cytosolic kinase BIK1 interacts with and phosphorylates the N-terminal cytosolic loop of OSCA1.3 within minutes of treatment with the peptidic PAMP flg22, which is derived from bacterial flagellin. Genetic and electrophysiological data reveal that OSCA1.3 is permeable to Ca2+, and that BIK1-mediated phosphorylation on its N terminus increases this channel activity. Notably, OSCA1.3 and its phosphorylation by BIK1 are critical for stomatal closure during immune signalling, and OSCA1.3 does not regulate stomatal closure upon perception of abscisic acid—a plant hormone associated with abiotic stresses. This study thus identifies a plant Ca2+ channel and its activation mechanisms underlying stomatal closure during immune signalling, and suggests specificity in Ca2+ influx mechanisms in response to different stresses.
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影响因子：
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作者：
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通讯作者：
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