Highly Aligned Nanofibrous Scaffold Derived from Decellularized Human Fibroblasts.
Highly Aligned Nanofibrous Scaffold Derived from Decellularized Human Fibroblasts.
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DOI：
10.1002/adfm.201303460
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发表时间：
2014-05-28
影响因子：
19
通讯作者：
Zhao, Feng
中科院分区：
文献类型：
--
作者：
Xing, Qi;Vogt, Caleb;Leong, Kam W.;Zhao, Feng
天然组织具有高度组织化的纳米纤维细胞外基质（ECM），其指导细胞分布和功能。本研究的目的是创造一种纯天然的，均匀的，高度对齐的纳米纤维ECM支架的潜在组织工程应用。合成纳米光栅（深度130 nm）用于指导人真皮成纤维细胞的生长长达8周，产生具有高度对齐的细胞和ECM纳米纤维的均匀70 μ m厚的成纤维细胞片。在从分离的成纤维细胞片层去除细胞组分后，产生具有直径为78 ± 9nm的均匀排列的纳米纤维的天然ECM支架。由于弹性蛋白纤维的保留，支架的弹性模量在去细胞化过程后保持良好。重新种植的人间充质干细胞（hMSCs）显示出支架在引导和支持细胞排列和沿着下方纤维增殖方面的优异能力。支架的生物相容性通过接种巨噬细胞的体外炎症测定进一步检查。与其未对齐的对应物相比，对齐的ECM支架诱导显著较低的免疫应答，如通过从巨噬细胞分泌的促炎细胞因子所检测到的。排列的纳米纤维ECM支架在工程化组织中具有巨大的潜力。
Native tissues are endowed with a highly organized nanofibrous extracellular matrix (ECM) that directs cellular distribution and function. The objective of this study is to create a purely natural, uniform, and highly aligned nanofibrous ECM scaffold for potential tissue engineering applications. Synthetic nanogratings (130 nm in depth) were used to direct the growth of human dermal fibroblasts for up to 8 weeks, resulting in a uniform 70 μm–thick fibroblast cell sheet with highly aligned cells and ECM nanofibers. A natural ECM scaffold with uniformly aligned nanofibers of 78 ± 9 nm in diameter was generated after removing the cellular components from the detached fibroblast sheet. The elastic modulus of the scaffold was well maintained after the decellularization process because of the preservation of elastin fibers. Reseeding human mesenchymal stem cells (hMSCs) showed the excellent capacity of the scaffold in directing and supporting cell alignment and proliferation along the underlying fibers. The scaffold’s biocompatibility was further examined by an in vitro inflammation assay with seeded macrophages. The aligned ECM scaffold induced a significantly lower immune response compared to its unaligned counterpart, as detected by the pro-inflammatory cytokines secreted from macrophages. The aligned nanofibrous ECM scaffold holds great potential in engineering organized tissues.
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影响因子：
14
作者：
Chen, Sulin;Jones, Jacqueline A.;Xu, Yongan;Low, Hong-Yee;Anderson, James M.;Leong, Kam W.
通讯作者：
Leong, Kam W.
影响因子：
4.8
作者：
L'Heureux, N;Pâquet, S;Auger, FA
通讯作者：
Auger, FA
影响因子：
9.7
作者：
Franz, Sandra;Allenstein, Francie;Simon, Jan C.
通讯作者：
Simon, Jan C.
影响因子：
9.7
作者：
Koutsopoulos, Sotirios;Zhang, Shuguang
通讯作者：
Zhang, Shuguang
影响因子：
3.3
作者：
MIJAILOVICH, SM;STAMENOVIC, D;FREDBERG, JJ
通讯作者：
FREDBERG, JJ