Calcium Imaging for Detection and Estimation of Spike Activities in Aplysia Neurons
Calcium Imaging for Detection and Estimation of Spike Activities in Aplysia Neurons
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用于检测和估计海兔神经元尖峰活动的钙成像
DOI：
10.2108/zsj.18.631
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发表时间：
2001
期刊：
影响因子：
8.8
通讯作者：
T. Nagahama
中科院分区：
文献类型：
--
作者：
R. Yoshida;A. Iwamoto;T. Nagahama
摘要：探讨钙显像技术是否适用于大鼠广泛性神经元峰活性的检测。膜透性乙酰氧基甲基（AM）类型和逆行标记不能用于将钙敏感染料加载到许多神经元中，然而，用含有高浓度染料溶液的微电极依次刺穿每个细胞体可以在相对较短的时间内将染料离子透入许多大神经元，并在很短的时间内将染料离子透入几个小神经元中。尖峰活动仅在细胞体中诱导荧光增加，而无尖峰的去极化则在轴突丘中诱导荧光增加。在细胞体中，荧光增加的斜率几乎与峰值频率相关，而其周期几乎与放电持续时间相对应。因此，可以精确地检测多个神经元节律放电反应的相位关系。此外，对于不适合用微电极长时间记录的小神经元，可以长时间重复获得稳定的响应。去除细胞外Ca2+完全抑制了细胞内荧光的增加，硝苯地平的应用部分减少了荧光的增加，这表明l型通道在广布神经元中的分布有所贡献。
Abstract We explored whether the calcium imaging can be applicable to detection of spike activity of Aplysia wide-spread neurons. Well-used procedures, the membrane permeable acetoxymethyl (AM) types and the retrograde labeling could not be used for loading the calcium sensitive dye into many neurons, however, impalement of each cell body with a microelectrode containing high concentration of the dye solution in turn could load the dye iontophoretically into many large neurons in a relatively short time and into several small neurons in a very short time. Spike activity just induced a fluorescent increase in the cell body while depolarization without spikes induced it in the axon hillock. In the cell body the slope of the fluorescent increase was almost relative to the spike frequency while the period of it almost corresponded to the firing duration. Therefore, the phase relationship of the rhythmic firing responses in plural neurons could be precisely detected. Moreover stable responses could be repeatedly obtained for a long time from small neurons unsuitable for a long time recording with microelectrodes. Removal of the extracellular Ca2+ completely suppressed the spike-induced increase in fluorescence in the cell body and application of nifedipine partly reduced it, suggesting contribution of L-type channels distributing in Aplysia wide-spread neurons.
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影响因子：
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