Differentiation of rat osteoblast-like cells in monolayer and micromass cultures.
Differentiation of rat osteoblast-like cells in monolayer and micromass cultures.
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大鼠成骨细胞样细胞在单层和微团培养物中的分化。
DOI：
10.22203/ecm.v003a03
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发表时间：
2002
影响因子：
3.1
通讯作者：
I. Gerber
中科院分区：
文献类型：
--
作者：
Iolo A. ap Gwynn;I. Gerber
在膜内成骨过程中，成骨前细胞凝聚、分化为成骨细胞并沉积骨基质。我们比较了大鼠颅骨成骨样细胞接种成微团和单层细胞的分化过程，前者模拟了体内凝聚过程。在1、2和3周时，通过光镜（碱性磷酸酶活性、矿化）、透射电镜和生化（胶原分型、碱性磷酸酶活性、蛋白质和DNA含量）对细胞进行形态学分析。单层接种的细胞在培养期间形成碱性磷酸酶阳性和矿化结节。作为微团接种的细胞形成一个由较小结节组成的大矿化区。两种培养体系的细胞超微结构均具有成骨细胞和骨细胞的典型特征。培养1周后发现单层和微团培养的主要差异。作为微团接种的细胞形成多层细胞。细胞质中含有内质网、线粒体、囊泡和核糖体。膜褶皱及细胞外基质中胶原原纤维丰富。这与单层培养的细胞形成对比，细胞外基质中几乎没有胶原原纤维。生物化学数据也证实了微团对分化的促进作用，在培养期间，微团的DNA含量低于单层培养。这些结果表明，与单层培养相比，微团培养能促进体外培养大鼠成骨样细胞的分化。
During intramembranous bone formation, preosteoblasts condense, differentiate into osteoblasts and deposit bone matrix. We compared the differentiation process of rat calvarial osteoblast-like cells inoculated as micromasses, which mimic the in vivo condensation process, with cells inoculated as monolayers. The cells were analysed morphologically at 1,2 and 3 weeks by light microscopy (alkaline phosphatase activity, mineralization), by transmission electron microscopy, and biochemically (collagen typing, alkaline phosphatase activity, protein and DNA content). The cells inoculated as monolayers formed alkaline phosphatase positive and mineralized nodules during the culture period. The cells inoculated as a micromass formed a large mineralized area consisting of smaller nodules. The ultrastructure of the cells in both culture systems showed the typical features of osteoblasts and osteocytes. The main difference between monolayer and micromass cultures was found after 1 week in culture. The cells inoculated as a micromass formed a multilayer of cells. The cytoplasm contained rER, mitochondria, vesicles and ribosomes. There were abundant collagen fibrils in membrane folds and in the extracellular matrix. This was in contrast to the cells in monolayer culture which showed hardly any collagen fibrils in the extracellular matrix. The promotion of the differentiation was also confirmed by biochemical data showing that the DNA content was lower in the micromass than in the monolayer cultures during the culture period. These results show that micromass, as compared to monolayer, culture promotes the differentiation of rat osteoblast-like cells in vitro.
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DOI：
10.1152/ajpcell.2000.278.2.c315
发表时间：
2000-02-01
影响因子：
5.5
作者：
Donahue, HJ;Li, ZY;Yellowley, CE
通讯作者：
Yellowley, CE
影响因子：
4.1
作者：
Schiller, PC;D'Ippolito, G;Howard, GA
通讯作者：
Howard, GA
影响因子：
3.3
作者：
Lecanda, F;Towler, DA;Civitelli, R
通讯作者：
Civitelli, R
影响因子：
2.7
作者：
GERSTENFELD, LC;CHIPMAN, SD;LIAN, JB
通讯作者：
LIAN, JB
耦合不足的成骨细胞网络：磁场和电场暴露的差异效应。
影响因子：
4.1
作者：
VanderMolen,MA;Donahue,HJ;Rubin,CT;McLeod,KJ
通讯作者：
McLeod,KJ