Effects of chloroquine on the feeding mechanism of the intraerythrocytic human malarial parasite Plasmodium falciparum.
Effects of chloroquine on the feeding mechanism of the intraerythrocytic human malarial parasite Plasmodium falciparum.
复制标题
氯喹对红细胞内人类疟疾寄生虫恶性疟原虫摄食机制的影响。
DOI：
10.1111/j.1550-7408.1984.tb02981.x
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
H. Ginsburg
中科院分区：
文献类型：
--
作者：
A. Yayon;R. Timberg;Shoshana Friedman;H. Ginsburg
恶性疟原虫在体外培养的人红细胞的超微结构研究揭示了新的特点，寄生虫的喂养机制。成熟的滋养体和寄生体通过内吞作用占据一部分宿主细胞质，内吞作用仅限于胞孔，并涉及寄生虫和寄生虫膜的内陷。所产生的内吞囊泡，由两个同心膜包围，迁移到中央食物液泡膜。内吞囊泡的外膜与食物泡的外膜并列，导致由单个膜包围的囊泡内化到液泡空间中，在那里它们被迅速降解。我们从这一系列事件中得出结论，内吞小泡与食物泡融合。用治疗浓度的氯喹处理感染的细胞抑制了进食过程的最后一步，即液泡降解。这表现为由单个膜包围的完整囊泡在液泡空间内的积累。这些发现的影响氯喹的抗疟活性进行了讨论。
Ultrastructural investigations of P. falciparum cultivated in vitro in human erythrocytes revealed new features of the feeding mechanism of the parasite. Mature trophozoites and schizonts take up a portion of the host cytosol by endocytosis which is restricted to cytostomes and which involves the invagination of both parasitophorous and parasite membranes. The resulting endocytic vesicles, surrounded by two concentric membranes, migrate towards the central food vacuole membrane. The external membrane of the endocytic vesicles apposes that of the food vacuole, leading to the internalization of vesicles bounded by a single membrane into the vacuole space where they are rapidly degraded. We conclude from this sequence of events that endocytic vesicles fuse with the food vacuole. Treatment of infected cells with therapeutic concentrations of chloroquine inhibited the last step of the feeding process, i.e. vacuolar degradation. This was manifested by the accumulation within the vacuolar space of intact vesicles bounded by single membranes. The implications of these findings for the antimalarial activity of chloroquine are discussed.
影响因子：
2.1
作者：
PFALLER, MA;KROGSTAD, DJ;PARQUETTE, AR
通讯作者：
PARQUETTE, AR