Drosophila female reproductive glands contribute to mating plug composition and the timing of sperm ejection.
Drosophila female reproductive glands contribute to mating plug composition and the timing of sperm ejection.
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DOI：
10.1098/rspb.2021.2213
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发表时间：
2022-02-09
期刊：
影响因子：
--
通讯作者：
Dorus S
中科院分区：
文献类型：
--
作者：
McDonough-Goldstein CE;Pitnick S;Dorus S
影响雌性再交配和竞争性受精的生殖特征随着性选择和性冲突而迅速进化。在不同的动物类群中观察到的这样一个特征是，在交配期间或交配后不久，雌性生殖道（FRT）内会形成结构塞。在果蝇中，雄性精液在雌性囊内形成交配塞，这已被证明会影响精子进入储存和雌性再交配的潜伏期。塞子的加工，包括其最终从雌性生殖道中排出，影响其配偶的竞争性受精成功率，并且是由雌性×雄性基因型相互作用介导的。然而，女性对树塞形成和加工的贡献受到的关注有限。使用缺乏腺体 FRT 组织的发育突变体，我们发现这些组织对于交配栓弹出至关重要。我们进一步利用蛋白质组学来证明雌性腺蛋白，尤其是蛋白水解酶，有助于交配栓的组成，并对交配栓的形成和组成产生广泛的影响。这些表型和分子数据共同确定了女性对两性相互作用的贡献，这是交配后性选择的潜在机制。
Reproductive traits that influence female remating and competitive fertilization rapidly evolve in response to sexual selection and sexual conflict. One such trait, observed across diverse animal taxa, is the formation of a structural plug inside the female reproductive tract (FRT), either during or shortly after mating. In Drosophila melanogaster, male seminal fluid forms a mating plug inside the female bursa, which has been demonstrated to influence sperm entry into storage and latency of female remating. Processing of the plug, including its eventual ejection from the female's reproductive tract, influences the competitive fertilization success of her mates and is mediated by female × male genotypic interactions. However, female contributions to plug formation and processing have received limited attention. Using developmental mutants that lack glandular FRT tissues, we reveal that these tissues are essential for mating plug ejection. We further use proteomics to demonstrate that female glandular proteins, and especially proteolytic enzymes, contribute to mating plug composition and have a widespread impact on plug formation and composition. Together, these phenotypic and molecular data identify female contributions to intersexual interactions that are a potential mechanism of post-copulatory sexual selection.
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