Functional conservation of DNA methylation in the pea aphid and the honeybee.
Functional conservation of DNA methylation in the pea aphid and the honeybee.
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DOI：
10.1093/gbe/evq057
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发表时间：
2010
影响因子：
3.3
通讯作者：
Goodisman MA
中科院分区：
文献类型：
--
作者：
Hunt BG;Brisson JA;Yi SV;Goodisman MA
DNA甲基化是一种基本的表观遗传标记，已知对各种动物分类群的基因调控具有广泛的影响。比较基因组分析可以通过鉴定甲基化基因和其他基因组区域的保守特征来帮助阐明DNA甲基化的功能。在这项研究中，我们使用计算方法来区分豌豆蚜虫Acyrthosiphon pisum中重度甲基化标记的基因和轻度甲基化或无甲基化标记的基因。我们通过与蜜蜂（Apis） mellifera进行比较分析，研究了这两个类别是否具有不同的进化历史和功能角色。我们发现，pisum和aps . mellifera的高甲基化同源基因表现出更大的甲基化状态守恒，这表明祖先物种的高甲基化基因可能随着时间的推移保持高度甲基化。我们还发现，甲基化基因倾向于表现出与未甲基化基因不同的进化速度。此外，与相对未甲基化的基因不同，甲基化靶向的基因在特定的生物过程中富集。最后，甲基化基因在两种物种的交替表型中优先普遍表达，而缺乏甲基化特征的基因则优先与条件特异性基因表达相关。总的来说，我们的分析支持具有类似甲基化系统的昆虫DNA甲基化的保守作用。
DNA methylation is a fundamental epigenetic mark known to have wide-ranging effects on gene regulation in a variety of animal taxa. Comparative genomic analyses can help elucidate the function of DNA methylation by identifying conserved features of methylated genes and other genomic regions. In this study, we used computational approaches to distinguish genes marked by heavy methylation from those marked by little or no methylation in the pea aphid, Acyrthosiphon pisum. We investigated if these two classes had distinct evolutionary histories and functional roles by conducting comparative analysis with the honeybee, Apis (Ap.) mellifera. We found that highly methylated orthologs in A. pisum and Ap. mellifera exhibited greater conservation of methylation status, suggesting that highly methylated genes in ancestral species may remain highly methylated over time. We also found that methylated genes tended to show different rates of evolution than unmethylated genes. In addition, genes targeted by methylation were enriched for particular biological processes that differed from those in relatively unmethylated genes. Finally, methylated genes were preferentially ubiquitously expressed among alternate phenotypes in both species, whereas genes lacking signatures of methylation were preferentially associated with condition-specific gene expression. Overall, our analyses support a conserved role for DNA methylation in insects with comparable methylation systems.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
蜜蜂转录组的表观遗传调控：揭示甲基化基因的本质。
影响因子：
4.4
作者：
Foret S;Kucharski R;Pittelkow Y;Lockett GA;Maleszka R
通讯作者：
Maleszka R
影响因子：
14.9
作者：
BIRD, AP
通讯作者：
BIRD, AP
影响因子：
1.9
作者：
SMITH, MAH;MACKAY, PA
通讯作者：
MACKAY, PA
影响因子：
3.7
作者：
Maleszka, Ryszard
通讯作者：
Maleszka, Ryszard