The Cis-Regulatory Code for Kelch-like 21/30 Specific Expression in Ciona robusta Sensory Organs.
The Cis-Regulatory Code for Kelch-like 21/30 Specific Expression in Ciona robusta Sensory Organs.
复制标题
DOI：
10.3389/fcell.2020.569601
复制
发表时间：
2020
影响因子：
5.5
通讯作者：
Ristoratore F
中科院分区：
文献类型：
--
作者：
Coppola U;Kamal AK;Stolfi A;Ristoratore F
被囊状乔纳罗布斯塔是研究神经系统进化的一个新兴模式系统。由于它们的小胚胎和紧凑的基因组，像乔纳罗布斯塔这样的被囊类动物，在理解不同神经细胞之间的细胞特异性基因谱系的遗传电路方面具有巨大的潜力。它们的简单幼虫有一个感觉囊，由两个有色的感觉器官--眼球和耳石组成。我们在这里重点研究了Klhl21/30，这是一个属于Kelch家族的基因，在Ciona robusta中，它开始在有色细胞前体中表达，并在胚胎发生期间特异性地维持在耳石前体中。进化分析表明，Klhl21/30在所有脊索动物中都是保守的。顺式调控分析和潜在上游因子的CRISPR/Cas9突变表明，Klhl21/30的表达受成纤维细胞生长因子信号下游的三个转录因子MITF、DMRT和MSX的共同作用控制。MITF的核心作用与其作为脊椎动物色素细胞发育基本调节因子的功能是一致的。此外，我们的结果揭示了DMRT和MSX作为转录共激活因子在乔纳耳石中的新功能。
The tunicate Ciona robusta is an emerging model system to study the evolution of the nervous system. Due to their small embryos and compact genomes, tunicates, like Ciona robusta, have great potential to comprehend genetic circuitry underlying cell specific gene repertoire, among different neuronal cells. Their simple larvae possess a sensory vesicle comprising two pigmented sensory organs, the ocellus and the otolith. We focused here on Klhl21/30, a gene belonging to Kelch family, that, in Ciona robusta, starts to be expressed in pigmented cell precursors, becoming specifically maintained in the otolith precursor during embryogenesis. Evolutionary analyses demonstrated the conservation of Klhl21/30 in all the chordates. Cis-regulatory analyses and CRISPR/Cas9 mutagenesis of potential upstream factors, revealed that Klhl21/30 expression is controlled by the combined action of three transcription factors, Mitf, Dmrt, and Msx, which are downstream of FGF signaling. The central role of Mitf is consistent with its function as a fundamental regulator of vertebrate pigment cell development. Moreover, our results unraveled a new function for Dmrt and Msx as transcriptional co-activators in the context of the Ciona otolith.
影响因子：
3.7
作者：
Sordino P;Andreakis N;Brown ER;Leccia NI;Squarzoni P;Tarallo R;Alfano C;Caputi L;D'Ambrosio P;Daniele P;D'Aniello E;D'Aniello S;Maiella S;Miraglia V;Russo MT;Sorrenti G;Branno M;Cariello L;Cirino P;Locascio A;Spagnuolo A;Zanetti L;Ristoratore F
通讯作者：
Ristoratore F