Generation of an isogenic collection of yeast actin mutants and identification of three interrelated phenotypes.
Generation of an isogenic collection of yeast actin mutants and identification of three interrelated phenotypes.
复制标题
酵母肌动蛋白突变体的同基因集合的产生和三种相互关联的表型的鉴定。
DOI：
10.1093/genetics/157.2.533
复制
发表时间：
2001
期刊：
影响因子：
3.3
通讯作者：
Adams,A
中科院分区：
文献类型：
--
作者：
Whitacre,J;Davis,D;Toenjes,K;Brower,S;Adams,A
大量的酵母肌动蛋白突变先前已被分离出来，并用于肌动蛋白细胞骨架功能的许多研究。然而，各种突变一直在同源，而不是同基因，背景，使其难以比较的微妙的表型，这些突变体的特点。因此，我们在同基因背景中放置了27个突变。我们使用这些突变体的一个子集来比较不同的肌动蛋白等位基因在含NaCl的培养基上的孢子形成、内吞和生长中的缺陷程度。我们发现这三种表型高度相关。相关性是特定的，而不仅仅是一般生长缺陷的反映，因为表型与正常条件下的生长速率不相关。值得注意的是，这些肌动蛋白突变体表现出最严重的表型，在所有三个过程中改变了残基，集群到一个小区域的肌动蛋白晶体结构，以前定义为fimlobulin（Sac6p）结合位点。我们研究了内吞作用和盐上生长之间的关系，发现将野生型或肌动蛋白突变体细胞转移到高盐环境会降低α因子内化的速率。这些结果表明，肌动蛋白突变体可能无法生长的盐，因为添加剂的内吞缺陷（由于突变和盐）。
A large collection of yeast actin mutations has been previously isolated and used in numerous studies of actin cytoskeletal function. However, the various mutations have been in congenic, rather than isogenic, backgrounds, making it difficult to compare the subtle phenotypes that are characteristic of these mutants. We have therefore placed 27 mutations in an isogenic background. We used a subset of these mutants to compare the degree to which different actin alleles are defective in sporulation, endocytosis, and growth on NaCl-containing media. We found that the three phenotypes are highly correlated. The correlations are specific and not merely a reflection of general growth defects, because the phenotypes are not correlated with growth rates under normal conditions. Significantly, those actin mutants exhibiting the most severe phenotypes in all three processes have altered residues that cluster to a small region of the actin crystal structure previously defined as the fimbrin (Sac6p)-binding site. We examined the relationship between endocytosis and growth on salt and found that shifting wild-type or actin mutant cells to high salt reduces the rate of α-factor internalization. These results suggest that actin mutants may be unable to grow on salt because of additive endocytic defects (due to mutation and salt).
登录
查看更多内容
DOI：
10.1083/jcb.135.4.965
发表时间：
1996-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Rivero F;Furukawa R;Noegel AA;Fechheimer M
通讯作者：
Fechheimer M
子结构域 3 和 4 (L266D) 之间的“疏水塞”发生突变的酵母肌动蛋白表现出冷敏聚合缺陷
影响因子：
7.8
作者：
Xin Chen;R. Cook;P. Rubenstein
通讯作者：
P. Rubenstein
通过在酵母中形成新的蛋白质-蛋白质相互作用来进行等位基因特异性抑制。
影响因子：
3.3
作者：
Sandrock,TM;O'Dell,JL;Adams,AE
通讯作者：
Adams,AE
酵母中菌丝蛋白 (Sac6p) 过度表达的抑制子分析。
影响因子：
3.3
作者：
Sandrock,TM;Brower,SM;Toenjes,KA;Adams,AE
通讯作者：
Adams,AE
影响因子：
5.6
作者：
CARPENTIER, JL;SAWANO, F;ORCI, L
通讯作者：
ORCI, L