Bacteriophage endolysins as novel antimicrobials.
Bacteriophage endolysins as novel antimicrobials.
复制标题
DOI：
10.2217/fmb.12.97
复制
发表时间：
2012-10
影响因子：
3.1
通讯作者：
Loessner MJ
中科院分区：
文献类型：
--
作者：
Schmelcher M;Donovan DM;Loessner MJ
内溶素是噬菌体在其复制周期结束时使用的酶，用于从内部降解细菌宿主的肽聚糖，导致细胞裂解并释放子代病毒颗粒。由于革兰氏阳性细菌细胞壁中没有外膜，内溶素可以进入肽聚糖并在外部应用时破坏这些生物体，使它们成为有趣的抗菌候选者，特别是考虑到细菌耐药性不断增加。本文回顾了这些酶的模块化结构，其中细胞壁结合和催化功能是分开的，以及它们的作用机制、裂解活性和作为抗菌剂的潜力。它特别关注分子工程作为优化特定应用的细胞内溶素的手段，强调了可能使这些蛋白质对革兰氏阴性和细胞内病原体具有活性的新进展，并总结了细胞内溶素在医学、食品安全、农业和生物技术领域的最新应用。
Endolysins are enzymes used by bacteriophages at the end of their replication cycle to degrade the peptidoglycan of the bacterial host from within, resulting in cell lysis and release of progeny virions. Due to the absence of an outer membrane in the Gram-positive bacterial cell wall, endolysins can access the peptidoglycan and destroy these organisms when applied externally, making them interesting antimicrobial candidates, particularly in light of increasing bacterial drug resistance. This article reviews the modular structure of these enzymes, in which cell wall binding and catalytic functions are separated, as well as their mechanism of action, lytic activity and potential as antimicrobials. It particularly focuses on molecular engineering as a means of optimizing endolysins for specific applications, highlights new developments that may render these proteins active against Gram-negative and intracellular pathogens and summarizes the most recent applications of endolysins in the fields of medicine, food safety, agriculture and biotechnology.
登录
查看更多内容
影响因子：
3.3
作者：
Celia, Laura K.;Nelson, Daniel;Kerr, David E.
通讯作者：
Kerr, David E.
DOI：
10.1073/pnas.91.9.4034
发表时间：
1994-04-26
影响因子：
11.1
作者：
CHENG, XD;ZHANG, X;STUDIER, FW
通讯作者：
STUDIER, FW
影响因子：
4
作者：
Briers, Y.;Walmagh, M.;Lavigne, R.
通讯作者：
Lavigne, R.
影响因子：
4.4
作者：
DEHART, HP;HEATH, HE;SLOAN, GL
通讯作者：
SLOAN, GL
影响因子：
2.4
作者：
Arnoldi, M;Fritz, M;Boulbitch, A
通讯作者：
Boulbitch, A