Expression analysis of Runx3 and other Runx family members during Xenopus development.
Expression analysis of Runx3 and other Runx family members during Xenopus development.
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DOI：
10.1016/j.gep.2010.04.004
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发表时间：
2010-06
期刊：
影响因子：
--
通讯作者：
Saint-Jeannet JP
中科院分区：
文献类型：
--
作者：
Park BY;Saint-Jeannet JP
Runx基因编码由高度保守的Runt DNA结合结构域定义的蛋白质家族。在几种生物中的研究表明，这些转录因子调节胚胎发育的多个方面，并负责几种人类疾病的发病机制。在这里，我们报告Runx 3在非洲爪蟾发育过程中的克隆和表达，并将其表达模式与其他Runx家族成员Runx 1和Runx 2以及Runx蛋白的强制性结合伴侣Cbfβ进行比较。使用原位杂交在整个胚胎和部分，我们表明，Runx 3与Runx 1在造血谱系和Rohon-Beard感觉神经元共表达。相反，Runx 3和Runx 2在颅面软骨成分中共表达。Runx 3还在神经源性基板的许多衍生物中显示出独特的表达结构域，包括前后和中侧线神经的神经节，以及三叉神经、舌咽神经、面神经和迷走神经的神经节。这些观察结果表明Runx 3在颅感觉神经元的发育中起关键作用，而在其他组织中，其与Runx 1或Runx 2的共表达可能意味着这些家族成员之间的功能冗余。
Runx genes encode a family of proteins defined by the highly conserved Runt DNA-binding domain. Studies in several organisms have shown that these transcription factors regulate multiple aspects of embryonic development and are responsible for the pathogenesis of several human diseases. Here we report the cloning and expression of Runx3 during Xenopus development and compare its expression pattern to other Runx family members, Runx1 and Runx2, and to Cbfβ, the obligatory binding-partner of Runx proteins. Using in situ hybridization in the whole embryo and on sections we show that Runx3 is co-expressed with Runx1 in the hematopoietic lineage and in Rohon-Beard sensory neurons. In contrast Runx3 and Runx2 are co-expressed in craniofacial cartilage elements. Runx3 shows also unique expression domains in a number of derivatives of the neurogenic placodes, including the ganglia of the anteroposterior and middle lateral line nerves, and ganglia of the trigeminal, glossopharyngeal, facial and vagal nerves. These observations suggest a critical role for Runx3 in the development of cranial sensory neurons, while in other tissues its co-expression with Runx1 or Runx2 may signify functional redundancy between these family members.
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