The DNA load of six high-risk human papillomavirus types and its association with cervical lesions.
The DNA load of six high-risk human papillomavirus types and its association with cervical lesions.
复制标题
六种高危人乳头瘤病毒类型的DNA负荷及其与宫颈病变的关联。
DOI：
10.1186/s12885-015-1126-z
复制
发表时间：
2015-03-05
期刊：
影响因子：
3.8
通讯作者：
Patarroyo MA
中科院分区：
文献类型：
--
作者：
Del Río-Ospina L;Soto-De León SC;Camargo M;Moreno-Pérez DA;Sánchez R;Pérez-Prados A;Patarroyo ME;Patarroyo MA
分析人乳头瘤病毒 (HPV) 病毒载量对于确定患宫颈癌 (CC) 的风险非常重要；迄今为止，大多数有关 HPV 病毒载量和宫颈病变的知识都与 HPV-16 有关。这项研究评估了哥伦比亚六种最流行的高危病毒类型的病毒载量与宫颈上皮内瘤变 (CIN) 频率之间的关联。该研究纳入了 114 名无 CIN 的女性和 59 名经阴道镜检查确诊为 CIN 的女性，她们在初次筛查中均通过常规 PCR 检测 HPV 感染呈阳性。对样本进行了六种高危 HPV 类型的检测，通过实时 PCR 确定病毒拷贝数。估算了粗略比值比和调整后比值比 (ORa)，以评估每种病毒类型的 DNA 载量与宫颈病变发生风险之间的关联。 CIN 患者中的 HPV-33 病毒载量最高，无病变患者中的 HPV-31 病毒载量最高（HPV 拷贝数为 9.33，四分位距 (IQR) 为 2.95；HPV 拷贝数为 9.41，IQR 为 2.58）。与 HPV-16 载量高的患者（2.62 ORa，1.08–6.35 95% CI）相比，低病毒载量的 HPV-16 患者（3.53 ORa，1.16–10.74 95% CI）病变更常见。 HPV-31 患者的高病毒载量与较低的 CIN 频率相关（0.34 ORa，0.15–0.78 95%CI）。 HPV DNA 载量与 CIN 频率之间的关联被认为具有类型特异性，并且可能取决于感染持续时间。该分析为了解 HPV DNA 载量对宫颈病变发展的影响提供了信息。
Analysing human papillomavirus (HPV) viral load is important in determining the risk of developing cervical cancer (CC); most knowledge to date regarding HPV viral load and cervical lesions has been related to HPV-16. This study evaluated the association between the viral load of the six most prevalent high-risk viral types in Colombia and cervical intraepithelial neoplasia (CIN) frequency. 114 women without CIN and 59 women having CIN confirmed by colposcopy, all of them positive by conventional PCR for HPV infection in the initial screening, were included in the study. Samples were tested for six high-risk HPV types to determine viral copy number by real-time PCR. Crude and adjusted odds ratios (ORa) were estimated for evaluating the association between each viral type’s DNA load and the risk of cervical lesions occurring. The highest viral loads were identified for HPV-33 in CIN patients and for HPV-31 in patients without lesions (9.33 HPV copies, 2.95 interquartile range (IQR); 9.41 HPV copies, 2.58 IQR). Lesions were more frequent in HPV-16 patients having a low viral load (3.53 ORa, 1.16–10.74 95%CI) compared to those having high HPV-16 load (2.62 ORa, 1.08–6.35 95%CI). High viral load in HPV-31 patients was associated with lower CIN frequency (0.34 ORa, 0.15–0.78 95%CI). An association between HPV DNA load and CIN frequency was seen to be type-specific and may have depended on the duration of infection. This analysis has provided information for understanding the effect of HPV DNA load on cervical lesion development.
登录
查看更多内容
影响因子：
8.8
作者：
通讯作者：
--
影响因子：
6.4
作者：
Moberg, M;Gustavsson, I;Gyllensten, U
通讯作者：
Gyllensten, U
影响因子：
5.6
作者：
Camargo, Milena;Soto-De Leon, Sara C.;Patarroyo, Manuel A.
通讯作者：
Patarroyo, Manuel A.
DOI：
10.1016/s0020-7292(00)00382-9
发表时间：
2001-02-01
影响因子：
3.8
作者：
Cronjé, HS;Cooreman, BF;Divall, PDJ
通讯作者：
Divall, PDJ
影响因子：
8.8
作者：
Munoz, N.;Hernandez-Suarez, G.;Mendez, F.;Molano, M.;Posso, H.;Moreno, V.;Murillo, R.;Ronderos, M.;Meijer, C.;Munoz, A.
通讯作者：
Munoz, A.