Super-Resolution Imaging of Higher-Order Chromatin Structures at Different Epigenomic States in Single Mammalian Cells.
Super-Resolution Imaging of Higher-Order Chromatin Structures at Different Epigenomic States in Single Mammalian Cells.
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DOI：
10.1016/j.celrep.2018.06.085
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发表时间：
2018-07-24
期刊：
影响因子：
8.8
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Xu J;Ma H;Jin J;Uttam S;Fu R;Huang Y;Liu Y
组蛋白修饰影响个体表观基因组状态和染色质环境下的高阶染色质结构以调节基因表达。然而，由不同组蛋白修饰形成的全基因组高阶染色质结构仍然很难表征。利用随机光学重建显微镜（STORM），我们在其表观基因组状态下表征了高阶染色质结构，在间期分为三种主要类型：组蛋白乙酰化标记形成空间分离的纳米簇，活性组蛋白甲基化标记形成空间分散的较大纳米结构域，抑制性组蛋白甲基化标记形成浓缩的大聚集体。这些独特的结构特征也在有丝分裂染色体中观察到。此外，活性组蛋白标记与不太紧凑的染色质相一致，并与其他活性标记和RNA聚合酶II（RNAP II）表现出更高程度的共定位，而抑制性标记与密集的染色质相一致，并且在空间上远离抑制性标记和活性RNAP II。总之，超分辨率成像揭示了三种不同的染色质结构在不同的表观基因组状态，这可能是空间协调影响转录。使用基于STORM的超分辨率显微镜，Xu等人发现，在各种表观基因组状态下的全基因组高阶染色质结构形成三种类型的不同结构：分离的纳米簇，分散的纳米结构域和紧凑的大聚集体。它们彼此之间的空间关系和RNA聚合酶II表明影响转录的空间协调。
Histone modifications influence higher-order chromatin structures at individual epigenomic states and chromatin environments to regulate gene expression. However, genome-wide higher-order chromatin structures shaped by different histone modifications remain poorly characterized. With stochastic optical reconstruction microscopy (STORM), we characterized the higher-order chromatin structures at their epigenomic states, categorized into three major types in interphase: histone acetylation marks form spatially segregated nanoclusters, active histone methylation marks form spatially dispersed larger nanodomains, and repressive histone methylation marks form condensed large aggregates. These distinct structural characteristics are also observed in mitotic chromosomes. Furthermore, active histone marks coincide with less compact chromatin and exhibit a higher degree of co-localization with other active marks and RNA polymerase II (RNAP II), while repressive marks coincide with densely packed chromatin and spatially distant from repressive marks and active RNAP II. Taken together, super-resolution imaging reveals three distinct chromatin structures at various epigenomic states, which may be spatially coordinated to impact transcription. Using STORM-based super-resolution microscopy, Xu et al. discover that genome-wide higher-order chromatin structures at various epigenomic states form three types of distinct structures: segregated nanoclusters, dispersed nanodomains, and compact large aggregates. Their spatial relationship with each other and RNA polymerase II suggests spatial coordination that impacts transcription.
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DOI：
10.1038/nrg3454
发表时间：
2013-06
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
--
影响因子：
17.1
作者：
Chen, Xuanze;Wei, Mian;Sun, Yujie
通讯作者：
Sun, Yujie
影响因子：
4.3
作者：
Caetano FA;Dirk BS;Tam JH;Cavanagh PC;Goiko M;Ferguson SS;Pasternak SH;Dikeakos JD;de Bruyn JR;Heit B
通讯作者：
Heit B
影响因子：
56.9
作者：
Bates, Mark;Huang, Bo;Zhuang, Xiaowei
通讯作者：
Zhuang, Xiaowei
影响因子：
2.3
作者：
Malkusch, Sebastian;Endesfelder, Ulrike;Heilemann, Mike
通讯作者：
Heilemann, Mike