A three-dimensional scaffold from decellularized human umbilical artery for bile duct reconstruction.
A three-dimensional scaffold from decellularized human umbilical artery for bile duct reconstruction.
复制标题
用于胆管重建的脱细胞人脐动脉三维支架。
DOI：
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复制
发表时间：
2019
影响因子：
0.8
通讯作者：
Z. Kakabadze
中科院分区：
文献类型：
--
作者：
K. Chakhunashvili;M. Kiladze;David G Chakhunashvili;L. Karalashvili;Z. Kakabadze
目的
该研究的主要目的是用脱细胞的人脐带动脉制造体外胆管等效物，并在保留奥狄括约肌的情况下用它来重建胆总管阻塞。
材料和方法
SDS和Triton X-100用于动脉的脱细胞化。同种异体分离的胆管细胞接种到脱细胞动脉的内表面上。进行实验研究，使用12头体重25-30 kg的雌雄家猪。他们被分成两组。所有动物均制作胆总管梗阻模型。第一组动物（n=6）未接受进一步治疗并接受观察。第二组动物（n=6）在初始干预后2天进行再次剖腹术，切开病变部位（2 cm），并用2- 2.5 cm的等同胆管重建缺损。最长观察期为84天。
结果
实验室、形态学和放射学研究显示与宿主有机体具有良好的整合。
讨论
胆道重建仍是肝外胆管手术的主要内容。这一事实促使本研究通过适当的实验室、形态学和放射学研究来评估这种新方法重建胆管的有效性。
结论
用所描述的方法获得的初步结果允许我们说，我们用脱细胞的人脐动脉和胆管细胞创建的胆管等同物可以成功地用于包含奥狄括约肌的胆管重建。
关键词
胆管梗阻，胆管重建，脱细胞，人脐动脉。
AIM
The main purpose of the study was to create in vitro bile duct equivalent out of decellularized human umbilical cord artery and use it to reconstruct common bile duct obstruction with preservation of sphincter of Oddi.
MATERIAL AND METHODS
SDS and Triton X-100 were used for decellularization of the artery. Allogeneic isolated cholangiocytes were seeded onto the inner surface of the decellularized artery. Experimental study was held and 12 domestic pigs of both sexes, weighing 25-30 kg were used. They were divided in equivalent two groups. Common bile duct obstruction model was created in all animals. Animals of the first group (n=6) received no further treatment and were under observation. Animals of the second group (n=6) underwent relaparotomy after two days of initial intervention, lesion site (2 cm) was incised and defect was reconstructed with the bile duct equivalent with the size of 2-2,5 cm. Maximum observation period was 84 days.
RESULTS
Laboratory, morphologic and radiologic investigations showed good integration with the host organism.
DISCUSSION
Bile duct reconstruction is still a major of HPB surgery. This fact prompted this study to assess the efficacy of the novel method for bile duct reconstruction the experimental study by using appropriate laboratory, morphologic and radiologic investigations.
CONCLUSION
Preliminary results obtained with the described method allows us to say that bile duct equivalent created by us with decellularized human umbilical artery and cholangiocytes can be successfully used for bile duct reconstruction with inclusion of the sphincter of oddi.
KEY WORDS
Bile Duct Obstruction, Bile Duct Reconstruction, Decellularization, Human Umbilical Artery.
DOI：
10.1002/jbm.b.33153
发表时间：
2015-01
期刊：
Journal of biomedical materials research. Part B, Applied biomaterials
影响因子：
--
作者：
Full SM;Delman C;Gluck JM;Abdmaulen R;Shemin RJ;Heydarkhan-Hagvall S
通讯作者：
Heydarkhan-Hagvall S
影响因子：
--
作者：
Fröhlich M;Grayson WL;Marolt D;Gimble JM;Kregar-Velikonja N;Vunjak-Novakovic G
通讯作者：
Vunjak-Novakovic G