Effect of fiber orientation of collagen-based electrospun meshes on human fibroblasts for ligament tissue engineering applications.
Effect of fiber orientation of collagen-based electrospun meshes on human fibroblasts for ligament tissue engineering applications.
复制标题
DOI：
10.1002/jbm.b.33153
复制
发表时间：
2015-01
期刊：
影响因子：
--
通讯作者：
Heydarkhan-Hagvall S
中科院分区：
文献类型：
--
作者：
Full SM;Delman C;Gluck JM;Abdmaulen R;Shemin RJ;Heydarkhan-Hagvall S
在过去的二十年里，聚乳酸-乙醇酸共聚物（PLGA）作为一种适用于组织工程和再生医学的生物相容性和可生物降解的聚合物受到了广泛的关注。在本研究中，我们研究了 PLGA、I 型胶原蛋白 (ColI) 和聚氨酯 (PU) 支架用于韧带组织再生的潜力。使用两种不同比例的 PLGA（50:50 和 85:15）来确定对机械拉伸性能和细胞粘附的影响。 PLGA(50:50)-ColI-PU 支架的杨氏模量、屈服拉伸应力和极限拉伸应变表现出与膝盖韧带相似的拉伸特性。而由 PLGA(85:15)-ColI-PU 组成的支架的拉伸性能低于韧带。此外，我们研究了纤维取向对机械性能的影响，结果表明，排列的纤维支架比随机纤维取向的支架表现出更高的拉伸性能。此外，人类成纤维细胞无需对这两类静电纺丝支架进行额外的表面修饰即可附着和增殖。总的来说，我们的研究证明了电纺 PLGA 支架作为再生医学合适候选者的有效性，能够被操纵并与其他聚合物结合以创建具有可调节拉伸特性的三维微环境来模拟天然组织。
Within the past two decades polylactic-co-glycolic acid (PLGA) has gained considerable attention as a biocompatible and biodegradable polymer that is suitable for tissue engineering and regenerative medicine. In this present study, we have investigated the potential of PLGA, collagen I (ColI), and polyurethane (PU) scaffolds for ligament tissue regeneration. Two different ratios of PLGA (50:50 and 85:15) were used to determine the effects on mechanical tensile properties and cell adhesion. The Young’s modulus, tensile stress at yield, and ultimate tensile strain of PLGA(50:50)-ColI-PU scaffolds demonstrated similar tensile properties to that of ligaments found in the knee. Whereas, scaffolds composed of PLGA(85:15)-ColI-PU had lower tensile properties than that of ligaments. Furthermore, we investigated the effect of fiber orientation on mechanical properties and our results indicate that aligned fiber scaffolds demonstrate higher tensile properties than scaffolds with random fiber orientation. Also, human fibroblasts attached and proliferated with no need for additional surface modifications to the presented electrospun scaffolds in both categories. Collectively, our investigation demonstrates the effectiveness of electrospun PLGA scaffolds as a suitable candidate for regenerative medicine, capable of being manipulated and combined with other polymers to create three-dimensional microenvironments with adjustable tensile properties to mimic native tissues.
登录
查看更多内容
影响因子：
5
作者：
Makadia HK;Siegel SJ
通讯作者：
Siegel SJ
影响因子：
3.1
作者：
Kim HS;Seon JK;Jo AR
通讯作者：
Jo AR
影响因子：
1.8
作者：
JONES, RS;NAWANA, NS;PATERSON, RS
通讯作者：
PATERSON, RS
影响因子：
4.1
作者：
Bashur, Chris A.;Shaffer, Robyn D.;Goldstein, Aaron S.
通讯作者：
Goldstein, Aaron S.
DOI：
10.1073/pnas.0608837104
发表时间：
2007-02-27
影响因子：
11.1
作者：
Cooper, James A., Jr.;Sahota, Janmeet S.;Laurencin, Cato T.
通讯作者：
Laurencin, Cato T.