Intramyocardial Injection of Pig Pluripotent Stem Cells Improves Left Ventricular Function and Perfusion: A Study in a Porcine Model of Acute Myocardial Infarction.
Intramyocardial Injection of Pig Pluripotent Stem Cells Improves Left Ventricular Function and Perfusion: A Study in a Porcine Model of Acute Myocardial Infarction.
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DOI：
10.1371/journal.pone.0066688
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Cao K
中科院分区：
文献类型：
--
作者：
Li X;Zhang F;Song G;Gu W;Chen M;Yang B;Li D;Wang D;Cao K
诱导多能干细胞（iPS）具有分化为各种类型的心血管细胞以修复受损心脏的潜力。基于iPS细胞的治疗的潜在治疗益处已经在心肌梗死（MI）的小动物模型中确立。我们假设猪iPS（piPS）细胞移植可能是MI的有效治疗方法。 在猪模型中左前降支动脉闭塞90分钟后，将未分化的piPS细胞或PBS注射到缺血心肌中。观察心功能、心肌灌注和细胞分化情况。在piPS细胞递送后一周，与假手术组相比，iPS组和PBS组中的总体左心室射血分数（LVEF）均显著降低（分别为p<0.05）。在piPS细胞递送后六周，iPS组的LVEF与PBS组相比显著改善（56.68%对50.93%，p = 0.04），但仍低于假手术组。 同样，与PBS注射相比，piPS细胞移植改善了局部灌注（19.67%与13.67%，p = 0.02）。 iPS组的梗死面积显著小于PBS组（12.04% vs. 15.98% p = 0.01）。 移植到心肌中的PiPS细胞可以分化成血管细胞，这导致梗死心脏中新血管的形成增加。在免疫抑制的猪AMI模型中，直接心肌内注射piPS细胞可以减小梗死面积并改善左心室功能和灌注。
Induced pluripotent stem (iPS) cells have the potential to differentiate to various types of cardiovascular cells to repair an injured heart. The potential therapeutic benefits of iPS cell based treatment have been established in small-animal models of myocardial infarction (MI). We hypothesize that porcine iPS (piPS) cell transplantation may be an effective treatment for MI. After a 90-minute occlusion of the left anterior descending artery in a porcine model, undifferentiated piPS cells or PBS were injected into the ischemic myocardium. Cardiac function, myocardial perfusion and cell differentiation were investigated. One week after piPS cell delivery, global left ventricular ejection fraction (LVEF) significantly decreased in both the iPS group and the PBS group compared to the Sham group (p<0.05, respectively). Six weeks after piPS cell delivery, LVEF of the iPS group significantly improved compared to the PBS group (56.68% vs. 50.93%, p = 0.04) but was still lower than the Sham group. Likewise, the piPS cell transplantation improved the regional perfusion compared to the PBS injection (19.67% vs. 13.67%, p = 0.02). The infarct area was significantly smaller in the iPS group than the PBS group (12.04% vs. 15.98% p = 0.01). PiPS cells engrafted into the myocardium can differentiate into vessel cells, which result in increased formation of new vessels in the infarcted heart. Direct intramyocardial injection of piPS cells can decrease infarct size and improve left ventricular function and perfusion for an immunosuppressed porcine AMI model.
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影响因子：
24
作者：
Dai, Bo;Huang, Wei;Xu, Meifeng;Millard, Ronald W.;Gao, Mei Hua;Hammond, H. Kirk;Menick, Donald R.;Ashraf, Muhammad;Wang, Yigang
通讯作者：
Wang, Yigang
影响因子：
4.8
作者：
Esteban, Miguel A.;Xu, Jianyong;Pei, Duanqing
通讯作者：
Pei, Duanqing
影响因子：
3.5
作者：
So, Kyoung Ha;Han, Young Joon;Park, Se Pill
通讯作者：
Park, Se Pill
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DOI：
10.1073/pnas.1018925108
发表时间：
2011-02-01
影响因子：
11.1
作者：
Tao, Zhengxian;Chen, Bo;Su, Hua
通讯作者：
Su, Hua
影响因子：
64.5
作者：
Takahashi, Kazutoshi;Yamanaka, Shinya
通讯作者：
Yamanaka, Shinya