Alternative splicing of OsLG3b controls grain length and yield in japonica rice.
Alternative splicing of OsLG3b controls grain length and yield in japonica rice.
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DOI：
10.1111/pbi.12903
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发表时间：
2018-02-25
影响因子：
13.8
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Yu J;Miao J;Zhang Z;Xiong H;Zhu X;Sun X;Pan Y;Liang Y;Zhang Q;Abdul Rehman RM;Li J;Zhang H;Li Z
水稻籽粒大小是决定产量的重要因素之一，受多个数量性状基因座（QTL）控制。在驯化过程中，与野生近缘种相比，现代栽培种对籽粒大小进行了密集的人工选择。在这里，我们报告的分子克隆和表征的OsLG 3b，一个QTL的粒长在热带粳稻中编码MADS-box转录因子1（OsMADS 1）。OsLG 3b区域的6个SNPs导致选择性剪接，在候选区域的关联分析中，这些SNPs与晶粒长度相关。定量PCR分析表明，OsLG 3b在穗部和种子发育期表达量较高。单倍型和渐渗区域分析表明，OsLG 3b的长粒等位基因可能是在热带粳稻驯化后产生的，并通过自然杂交和人工选择传播到亚种籼稻或温带粳稻中。因此，OsLG 3b是在水稻驯化和/或改良期间人类选择以适应热带地区的靶标。系统发育分析和系谱记录表明，OsLG 3b基因已被育种者利用，但该基因在籼稻粒长育种中仍具有很大的潜力。这些发现不仅有助于阐明水稻籽粒发育和驯化的分子基础，而且有助于水稻产量的遗传改良。
Grain size, one of the important components determining grain yield in rice, is controlled by the multiple quantitative trait loci (QTLs). Intensive artificial selection for grain size during domestication is evidenced in modern cultivars compared to their wild relatives. Here, we report the molecular cloning and characterization of OsLG3b, a QTL for grain length in tropical japonica rice that encodes MADS‐box transcription factor 1 (OsMADS1). Six SNPs in the OsLG3b region led to alternative splicing, which were associated with grain length in an association analysis of candidate region. Quantitative PCR analysis indicated that OsLG3b expression was higher during the panicle and seed development stages. Analysis of haplotypes and introgression regions revealed that the long‐grain allele of OsLG3b might have arisen after domestication of tropical japonica and spread to subspecies indica or temperate japonica by natural crossing and artificial selection. OsLG3b is therefore a target of human selection for adaptation to tropical regions during domestication and/or improvement of rice. Phylogenetic analysis and pedigree records showed that OsLG3b had been employed by breeders, but the gene still has much breeding potential for increasing grain length in indica. These findings will not only aid efforts to elucidate the molecular basis of grain development and domestication, but also facilitate the genetic improvement of rice yield.
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影响因子：
2.9
作者：
Lee, Shinyoung;Jeong, Dong-Hoon;An, Gynheung
通讯作者：
An, Gynheung
大米中的MADS-box基因家族：在生殖发育和压力期间，全基因组识别，组织和表达分析。
影响因子：
4.4
作者：
Arora, Rita;Agarwal, Pinky;Ray, Swatismita;Singh, Ashok Kumar;Singh, Vijay Pal;Tyagi, Akhilesh K.;Kapoor, Sanjay
通讯作者：
Kapoor, Sanjay
影响因子：
5.1
作者：
Lim, J;Moon, YH;Jang, SK
通讯作者：
Jang, SK
影响因子：
7
作者：
Hanada, Kousuke;Zhang, Xu;Shiu, Shin-Han
通讯作者：
Shiu, Shin-Han
影响因子：
7.4
作者：
Ferrario, S;Shchennikova, AV;Angenent, GC
通讯作者：
Angenent, GC