Identification of transcriptome induced in roots of maize seedlings at the late stage of waterlogging.
Identification of transcriptome induced in roots of maize seedlings at the late stage of waterlogging.
复制标题
淹水后期玉米幼苗根系诱导转录组鉴定
DOI：
10.1186/1471-2229-10-189
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发表时间：
2010-08-25
影响因子：
5.3
通讯作者：
Zheng Y
中科院分区：
文献类型：
--
作者：
Zou X;Jiang Y;Liu L;Zhang Z;Zheng Y
研究背景植物对低氧胁迫，特别是淹水胁迫的应答是通过改变转录和翻译来实现的。以往的研究多集中于揭示早期淹水胁迫下玉米根系的响应机制，而对淹水后期玉米根系基因转录谱的研究较少。耐涝性的遗传基础在很大程度上是未知的。本研究采用抑制性消减杂交技术，对耐涝自交系HZ 32根细胞在涝渍后期的转录组进行了分析。使用来自四个时间点的RNA群体的正向SSH文库（12 h、16 h、20 h和24 h）的表达水平进行分析，结果对768个cDNA克隆进行反向北方分析，发现大量基因在水稻中表达，并与水稻耐涝性相关基因进行了连锁分析。受水涝影响。共将465条EST组装成296个unigenes。生物信息学分析表明，这些基因参与了信号转导、蛋白质降解、离子转运、碳和氨基酸代谢、转录和翻译调控等复杂途径，可能在涝渍响应的后期发挥重要作用。大量的unigenes功能未知。约67%的unigenes可以在玉米基因组上对齐，其中63个共定位在已报道的QTLs.ConclusionThe玉米根系对淹水的后期反应涉及广泛的基因，主要与两个反应过程：防御在早期阶段和适应在后期阶段。信号转导在激活与植物耐涝机制相关的基因中起着关键作用。碳代谢与氨基酸代谢的相互影响揭示了氨基酸代谢在后期主要起两个作用：调节细胞质pH和通过破坏碳骨架提供能量。
BackgroundPlants respond to low oxygen stress, particularly that caused by waterlogging, by altering transcription and translation. Previous studies have mostly focused on revealing the mechanism of the response at the early stage, and there is limited information about the transcriptional profile of genes in maize roots at the late stage of waterlogging. The genetic basis of waterlogging tolerance is largely unknown. In this study, the transcriptome at the late stage of waterlogging was assayed in root cells of the tolerant inbred line HZ32, using suppression subtractive hybridization (SSH). A forward SSH library using RNA populations from four time points (12 h, 16 h, 20 h and 24 h) after waterlogging treatment was constructed to reveal up-regulated genes, and transcriptional and linkage data was integrated to identify candidate genes for waterlogging tolerance.ResultsReverse Northern analysis of a set of 768 cDNA clones from the SSH library revealed a large number of genes were up-regulated by waterlogging. A total of 465 ESTs were assembled into 296 unigenes. Bioinformatic analysis revealed that the genes were involved in complex pathways, such as signal transduction, protein degradation, ion transport, carbon and amino acid metabolism, and transcriptional and translational regulation, and might play important roles at the late stage of the response to waterlogging. A significant number of unigenes were of unknown function. Approximately 67% of the unigenes could be aligned on the maize genome and 63 of them were co-located within reported QTLs.ConclusionThe late response to waterlogging in maize roots involves a broad spectrum of genes, which are mainly associated with two response processes: defense at the early stage and adaption at the late stage. Signal transduction plays a key role in activating genes related to the tolerance mechanism for survival during prolonged waterlogging. The crosstalk between carbon and amino acid metabolism reveals that amino acid metabolism performs two main roles at the late stage: the regulation of cytoplasmic pH and energy supply through breakdown of the carbon skeleton.
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DOI：
10.1073/pnas.132259199
发表时间：
2002-07-09
影响因子：
11.1
作者：
Fu, HH;Dooner, HK
通讯作者：
Dooner, HK
影响因子：
4.3
作者：
FOX, GG;MCCALLAN, NR;RATCLIFFE, RG
通讯作者：
RATCLIFFE, RG
影响因子：
4.3
作者：
Dordas, C;Hasinoff, BB;Hill, RD
通讯作者：
Hill, RD
影响因子：
7.2
作者：
Dordas, C;Hasinoff, BB;Hill, RD
通讯作者：
Hill, RD
影响因子：
4.2
作者：
ERDMANN, B;HOFFMANN, P;WIEDENROTH, EM
通讯作者：
WIEDENROTH, EM