Growth factor functionalized biomaterial for drug delivery and tissue regeneration.
Growth factor functionalized biomaterial for drug delivery and tissue regeneration.
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DOI：
10.1177/0883911517705403
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发表时间：
2017-11
影响因子：
1.7
通讯作者：
Koria P
中科院分区：
文献类型：
--
作者：
Leonard A;Koria P
弹性蛋白样多肽（ELP）是一类天然衍生的非免疫原性生物材料，广泛应用于药物输送和组织工程。 ELP 经历温度介导的逆相变，这使得它们能够以相对简单的方式从细菌表达宿主中纯化。能够对 ELP 进行基因编码，可以掺入生物活性肽，从而使它们功能化。在这里，我们报告了一种具有生物活性的表皮生长因子-ELP (EGF-ELP) 融合蛋白的合成，该融合蛋白可以帮助伤口愈合。 EGF 通过诱导细胞增殖和迁移在伤口愈合中发挥着至关重要的作用。外源性EGF的使用在治疗急性伤口方面取得了成功，但在慢性伤口方面取得的成功相对较小，因为递送方法不能阻止其从应用部位扩散开。我们的数据表明EGF-ELP保留了EGF的生物活性和ELP的相变特性。此外，EGF-ELP 在接近生理温度下自组装的能力可以允许在伤口部位形成药物储库并最大限度地减少扩散，从而增加 EGF 的生物利用度并增强组织再生。
Elastin like polypeptides (ELPs) are a class of naturally derived and non-immunogenic biomaterials that are widely used in drug delivery and tissue engineering. ELPs undergo temperature-mediated inverse phase transitioning, which allows them to be purified in a relatively simple manner from bacterial expression hosts. Being able to genetically encode ELPs allows for the incorporation of bioactive peptides thereby functionalizing them. Here we report the synthesis of a biologically active epidermal growth factor-ELP (EGF-ELP) fusion protein that could aid in wound healing. EGF plays a crucial role in wound healing by inducing cell proliferation and migration. The use of exogenous EGF has seen success in the treatment of acute wounds, but has seen relatively minimal success in chronic wounds because the method of delivery does not prevent it from diffusing away from the application site. Our data shows that EGF-ELP retained the biological activity of EGF and the phase transitioning property of ELP. Furthermore, the ability of the EGF-ELP to self-assemble near physiological temperatures could allow for the formation of drug depots at the wound site and minimize diffusion, increasing the bioavailability of EGF and enhancing tissue regeneration.
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DOI：
10.1073/pnas.1009881108
发表时间：
2011-01-18
影响因子：
11.1
作者：
Koria, Piyush;Yagi, Hiroshi;Yarmush, Martin L.
通讯作者：
Yarmush, Martin L.
DOI：
10.1016/j.jconrel.2014.06.028
发表时间：
2014-09-28
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
MacEwan SR;Chilkoti A
通讯作者：
Chilkoti A
DOI：
10.1111/j.1524-4725.1992.tb03514.x
发表时间：
1992-07-01
期刊：
JOURNAL OF DERMATOLOGIC SURGERY AND ONCOLOGY
影响因子：
--
作者：
FALANGA, V;EAGLSTEIN, WH;CARSON, P
通讯作者：
CARSON, P
影响因子：
2.9
作者：
McCarthy, Bryce;Yuan, Yuan;Koria, Piyush
通讯作者：
Koria, Piyush
影响因子：
2.7
作者：
Alemdaroglu, C;Degim, Z;Erdogan, D
通讯作者：
Erdogan, D