Tannin Reduces the Incidence of Polyspermic Penetration in Porcine Oocytes.
Tannin Reduces the Incidence of Polyspermic Penetration in Porcine Oocytes.
复制标题
DOI：
10.3390/antiox11102027
复制
发表时间：
2022-10-14
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
Tannin (TA) improves porcine oocyte cytoplasmic maturation and subsequent embryonic development after in vitro fertilization (IVF). However, the mechanism through which TA blocks polyspermy after IVF remains unclear. Hence, the biological function of organelles (cortical granule [CG], Golgi apparatus, endoplasmic reticulum [ER], and mitochondria) and the incidence of polyspermic penetration were examined. We found no significant difference in oocyte nuclear maturation among the 1 µg/mL, 10 µg/mL TA, and control groups. Moreover, 100 μg/mL TA significantly reduced 1st polar body formation rate compared to the other groups. Additionally, 1 and 10 μg/mL TA significantly increased the protein levels of GDF9, BMP15, and CDK1 compared to the control and 100 μg/mL TA groups. Interestingly, 1 and 10 μg/mL TA improved the normal distribution of CGs, Golgi, ER, and mitochondria by upregulating organelle-related gene expression and downregulating ER stress (CHOP) gene expression. Simultaneously, 1 and 10 μg/mL TA significantly increased the proportion of normal fertilized oocytes (2 pronuclei; 2 PN) and blastocyst formation rate compared to the control, as well as that of 100 μg/mL TA after IVF by upregulating polyspermy-related genes. In conclusion, TA during IVM enhances 2PN and blastocyst formation rates by regulating organelles’ functions and activities.
登录
查看更多内容
两条途径调节皮质颗粒易位以防止小鼠卵母细胞的多精受精。
影响因子：
16.6
作者：
通讯作者：
--
影响因子：
4.6
作者：
Manzoor F;Nisa MU;Hussain HA;Ahmad N;Umbreen H
通讯作者：
Umbreen H
影响因子：
10.3
作者：
Lee, Sanghoon;Jin, Jun-Xue;Lee, Byeong Chun
通讯作者：
Lee, Byeong Chun
影响因子：
3.3
作者：
Jin, J. X.;Lee, S.;Lee, B. C.
通讯作者：
Lee, B. C.
影响因子：
3.6
作者：
Hao, Yanhong;Mathialagan, Nagappan;Prather, Randall S.
通讯作者：
Prather, Randall S.