Improved interface packing and design opportunities revealed by CryoEM analysis of a designed protein nanocage.
Improved interface packing and design opportunities revealed by CryoEM analysis of a designed protein nanocage.
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DOI：
10.1016/j.heliyon.2022.e12280
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发表时间：
2022-12
期刊：
影响因子：
4
通讯作者：
Gonen, Shane
中科院分区：
文献类型：
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作者：
McCarthy, Stephen;Gonen, Shane
对称蛋白质组装体在自然界中发挥着重要的作用，这使得它们成为一个有吸引力的工程目标。从头对称蛋白质复合物可以通过计算蛋白质设计来创建，以根据第一原理定制它们的特性，最近已经通过疏水相互作用将蛋白质组分聚集在一起来创建了几种蛋白质纳米笼。新开发的蛋白质的精确实验结构对于验证其设计，提高组装稳定性和定制下游应用至关重要。我们描述了纳米笼I3-01的CryoEM结构，总分辨率为3.5 μ m。I3-01，包括60个醛缩酶亚基排列与二十面体对称，已抵制高分辨率表征。改进后的结构与原始设计之间的一些关键差异被确定，例如疏水侧链的填充改善，从而深入了解I3-01对高分辨率平均的抵抗力。根据我们的分析，我们建议在新组件的设计和结构处理的重要因素。计算蛋白质设计;结构;纳米材料;对称性;二十面体。
Symmetric protein assemblies play important roles in nature which makes them an attractive target for engineering. De novo symmetric protein complexes can be created through computational protein design to tailor their properties from first principles, and recently several protein nanocages have been created by bringing together protein components through hydrophobic interactions. Accurate experimental structures of newly-developed proteins are essential to validate their design, improve assembly stability, and tailor downstream applications. We describe the CryoEM structure of the nanocage I3-01, at an overall resolution of 3.5 Å. I3-01, comprising 60 aldolase subunits arranged with icosahedral symmetry, has resisted high-resolution characterization. Some key differences between the refined structure and the original design are identified, such as improved packing of hydrophobic sidechains, providing insight to the resistance of I3-01 to high-resolution averaging. Based on our analysis, we suggest factors important in the design and structural processing of new assemblies. CryoEM; Computational protein design; Structure; Nanomaterial; Symmetry; Icosahedral.
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影响因子：
7.7
作者：
Nichols RJ;LaFrance B;Phillips NR;Radford DR;Oltrogge LM;Valentin-Alvarado LE;Bischoff AJ;Nogales E;Savage DF
通讯作者：
Savage DF
影响因子：
18
作者：
Alamos S;Reimer A;Niyogi KK;Garcia HG
通讯作者：
Garcia HG
影响因子：
17.1
作者：
Bruun TUJ;Andersson AC;Draper SJ;Howarth M
通讯作者：
Howarth M
影响因子：
14.9
作者：
Davis IW;Leaver-Fay A;Chen VB;Block JN;Kapral GJ;Wang X;Murray LW;Arendall WB 3rd;Snoeyink J;Richardson JS;Richardson DC
通讯作者：
Richardson DC
DOI：
10.1073/pnas.2206240119
发表时间：
2022-08-23
影响因子：
11.1
作者：
通讯作者：
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