Changes in phospholipid metabolism in exosomes of hormone-sensitive and hormone-resistant prostate cancer cells.
Changes in phospholipid metabolism in exosomes of hormone-sensitive and hormone-resistant prostate cancer cells.
复制标题
激素敏感和激素抵抗性前列腺癌细胞外泌体磷脂代谢的变化
DOI：
10.7150/jca.48906
复制
发表时间：
2021
影响因子：
3.9
通讯作者：
Liu T
中科院分区：
文献类型：
--
作者：
Yi X;Li Y;Hu X;Wang F;Liu T
背景：探索激素敏感和耐激素前列腺癌细胞外泌体的脂质变化，并开发出一种廉价且快速的技术，用于筛选前列腺癌的基于脂质的生物标志物。
方法：从LNCAP，PC3和DU-145细胞中提取外泌体，并使用高通量质谱法对其脂质组成进行定量分析。 LNCAP前列腺癌细胞释放的外泌体还使用基于聚乙烯甘氨酸（PEG）沉淀的修饰程序纯化。
结果：从LNCAP细胞中提取的外泌体比整个LNCAP细胞含有更高比例的磷脂酰胆碱，磷脂酰乙醇胺和磷脂酰肌醇脂质。溶血磷脂酰胆碱是体内磷脂酰胆碱代谢的有害中间产物，在LNCAP细胞中未发现，而是在外泌体中。来自LNCAP，PC3和DU-145前列腺癌细胞的外泌体的磷脂不同。在这些细胞中，涉及的主要脂质途径，即甘油磷脂代谢，自噬和铁凋亡途径也有所不同。通过这种改良的PEG沉淀技术分离的外泌体的纯度与使用商业试剂盒获得的外泌体相似。
结论：这项研究表明，磷脂酰胆碱及其有害产物溶物磷脂酰胆碱可能在激素对激素敏感的前列腺癌中起重要作用。激素敏感和耐激素的前列腺癌细胞中磷脂外泌体代谢发生了变化。 LPC自噬和铁吞作用的脂质途径可以充当治疗靶标。使用改良的PEG沉淀纯化前列腺癌细胞外泌体的可能性适合癌症筛查。
Background: To explore the changes in lipids in exosomes of hormone-sensitive and hormone-resistant prostate cancer cells and develop an inexpensive and rapid technique for screening lipid-based biomarkers of prostate cancer. Methods: Exosomes were extracted from LnCap, PC3 and DU-145 cells, and their lipid composition was analyzed quantitatively using high-throughput mass spectrometry. Exosomes released by LnCap prostate cancer cells were also purified using a modified procedure based on polyethylene glycol (PEG) precipitation. Results: Exosomes extracted from LnCap cells contained higher proportions of phosphatidyl choline, phosphatidyl ethanolamine and phosphatidyl inositol lipids than whole LnCap cells. Lysophosphatidylcholine, a harmful intermediate product of phosphatidylcholine metabolism in vivo, was not found in LnCap cells but in exosomes. Phospholipids were different in exosomes from LnCap, PC3 and DU-145 prostate cancer cells. The main lipid pathways involved, i.e., glycerophospholipid metabolism, autophagy, and ferroptosis pathways, were also different in these cells. Exosomes isolated by this modified PEG precipitation technique were similar in purity to those obtained using a commercial kit. Conclusions: This study demonstrates that phosphatidylcholine and its harmful product lysophosphatidylcholine may play important roles in hormone-sensitive prostate cancer. Phospholipid exosome metabolism was changed in hormone-sensitive and hormone-resistant prostate cancer cells. The LPC, lipid pathway of autophagy and ferroptosis may act as therapeutic targets. The possibility of purifying prostate cancer cell exosomes using modified PEG precipitation is suitable for cancer screening.
登录
查看更多内容
影响因子：
168.9
作者：
Grönberg, H
通讯作者：
Grönberg, H
影响因子：
3.8
作者：
Li J;Sherman-Baust CA;Tsai-Turton M;Bristow RE;Roden RB;Morin PJ
通讯作者：
Morin PJ
影响因子：
16.1
作者：
Butler LM;Perone Y;Dehairs J;Lupien LE;de Laat V;Talebi A;Loda M;Kinlaw WB;Swinnen JV
通讯作者：
Swinnen JV
影响因子：
16
作者：
Ludwig AK;De Miroschedji K;Doeppner TR;Börger V;Ruesing J;Rebmann V;Durst S;Jansen S;Bremer M;Behrmann E;Singer BB;Jastrow H;Kuhlmann JD;El Magraoui F;Meyer HE;Hermann DM;Opalka B;Raunser S;Epple M;Horn PA;Giebel B
通讯作者：
Giebel B
影响因子：
3.7
作者：
Duijvesz D;Burnum-Johnson KE;Gritsenko MA;Hoogland AM;Vredenbregt-van den Berg MS;Willemsen R;Luider T;Paša-Tolić L;Jenster G
通讯作者：
Jenster G