Temperature gradient chamber for relative growth rate analysis of yeast.
Temperature gradient chamber for relative growth rate analysis of yeast.
复制标题
用于酵母相对生长速率分析的温度梯度室。
DOI：
10.1006/abio.1998.2702
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发表时间：
1998
影响因子：
2.9
通讯作者：
C. Brunk
中科院分区：
文献类型：
--
作者：
G. Fogel;C. Brunk
相对生长通常用作区分突变型和野生型酵母或细菌菌株的表型测量。作为温度函数的差异生长是分析菌株之间差异的方便且准确的手段。两种菌株基因型的微小差异经常导致两种菌株作为温度的函数的差异生长。我们已经开发了一种腔室，用于在微量滴定板中沿着温度梯度同时生长多个菌株。使用图像分析来确定作为温度的函数的不同时间的菌落面积和数量。该室减少了所需的时间，并提高了测量生长随温度变化的准确性。这是通过允许沿着温度梯度测量相对生长而发生的，其中所有其他条件都是恒定的。两种酵母菌株（酿酒酵母）的生长温度依赖性的已知差异被用来证明该室的性能。
Relative growth is often used as a phenotypic measure to distinguish mutant and wild-type yeast or bacterial strains. Differential growth as a function of temperature is a convenient and accurate means of analyzing differences between strains. Slight differences in the genotypes of two strains frequently result in differential growth of the two strains as a function of temperature. We have developed a chamber for the simultaneous growth of multiple strains in microtiter plates along a temperature gradient. Image analysis was used to determine colony area and number at various times as a function of temperature. This chamber reduces the time required and increases the accuracy in measuring growth as a function of temperature. This occurs by allowing relative growth to be measured along a temperature gradient where all other conditions are constant. Two strains of yeast (Saccharomyces cerevisiae) with a known difference in temperature dependence of growth were used to demonstrate the performance of this chamber.
GCR3（一种参与酿酒酵母糖酵解基因表达的基因）的突变会抑制 hpr1 突变体的温度敏感生长。
影响因子：
3.3
作者：
Uemura,H;Pandit,S;Jigami,Y;Sternglanz,R
通讯作者：
Sternglanz,R
软琼脂中细胞数量和菌落直径之间的图像分析和数学相关性作为软琼脂生长的测量。
DOI：
10.1002/tcm.1770100408
发表时间：
1990
期刊：
Teratogenesis, carcinogenesis, and mutagenesis
影响因子：
--
作者：
Lewis,JC;Milo,GE
通讯作者：
Milo,GE