Global gene expression analysis of murine limb development.
Global gene expression analysis of murine limb development.
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DOI：
10.1371/journal.pone.0028358
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Loots GG
中科院分区：
文献类型：
--
作者：
Taher L;Collette NM;Murugesh D;Maxwell E;Ovcharenko I;Loots GG
有关基因表达阶段特异性变化的详细信息对于理解发育背后的基因调控网络以及有助于形态发生的各种信号转导途径至关重要。在这里，我们描述了早期小鼠肢体发育过程中的全局基因表达动态，当软骨、肌腱、肌肉、关节、脉管系统和神经被指定并建立四肢的肌肉骨骼系统时。我们使用全基因组微阵列来识别在前肢和后肢模式形成过程中肢体发育的 5 个阶段（E9.5 至 13.5）具有差异表达的基因。我们发现肢体形成的开始特征是转录因子的上调，随后在 E10.5 和 E11.5 期间基因大量激活，并在以后的时间点趋于平稳。在被鉴定为在肢体中显着上调的 3520 个基因中，我们发现~30% 是新的，这极大地扩展了可能在肢体中发挥作用的候选基因的库。分层和特定阶段的聚类确定了可能与肢体发育过程中的功能程序相关的表达谱，这些转录物的进一步表征将为特定组织模式化过程提供新的见解。在这里，我们首次对小鼠肢体发育过程中的发育调控基因进行了全面分析，并对控制肢体形态发生的表达动态提供了一些新的见解。
Detailed information about stage-specific changes in gene expression is crucial for understanding the gene regulatory networks underlying development and the various signal transduction pathways contributing to morphogenesis. Here we describe the global gene expression dynamics during early murine limb development, when cartilage, tendons, muscle, joints, vasculature and nerves are specified and the musculoskeletal system of limbs is established. We used whole-genome microarrays to identify genes with differential expression at 5 stages of limb development (E9.5 to 13.5), during fore- and hind-limb patterning. We found that the onset of limb formation is characterized by an up-regulation of transcription factors, which is followed by a massive activation of genes during E10.5 and E11.5 which levels off at later time points. Among the 3520 genes identified as significantly up-regulated in the limb, we find ∼30% to be novel, dramatically expanding the repertoire of candidate genes likely to function in the limb. Hierarchical and stage-specific clustering identified expression profiles that are likely to correlate with functional programs during limb development and further characterization of these transcripts will provide new insights into specific tissue patterning processes. Here, we provide for the first time a comprehensive analysis of developmentally regulated genes during murine limb development, and provide some novel insights into the expression dynamics governing limb morphogenesis.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
3.1
作者：
Malemud, CJ
通讯作者：
Malemud, CJ
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
4
作者：
Martin, G
通讯作者：
Martin, G
影响因子：
1.5
作者：
Qiu, Qiong;Chen, Haixu;Johnson, Randy L.
通讯作者：
Johnson, Randy L.