Stress-dependent opioid and adrenergic modulation of newly retrieved fear memory.
Stress-dependent opioid and adrenergic modulation of newly retrieved fear memory.
复制标题
DOI：
10.1016/j.nlm.2013.11.013
复制
发表时间：
2014-03
影响因子：
2.7
通讯作者：
Kirby LG
中科院分区：
文献类型：
--
作者：
Schneider AM;Simson PE;Daimon CM;Mrozewski J;Vogt NM;Keefe J;Kirby LG
最近在我们实验室对压力对恐惧记忆调节的影响的研究发现，内源性阿片类药物和肾上腺素能调节系统协同工作，分别限制了在轻度或强烈压力条件下巩固新获得的恐惧记忆的加强或减弱。本研究试图确定内源性阿片和肾上腺素能系统介导的类似应激依赖性调节是否发生在新检索的恐惧记忆的再巩固过程中。大鼠进行情境恐惧条件反射，24小时后再激活恐惧记忆；第二天进行记忆测试。在重新激活的过程中，通过不同的恐惧记忆回忆时间来控制压力。在第一个实验中，在不同持续时间（30或120秒）的再激活后立即给药载体或阿片受体阻滞剂纳洛酮。结果表明，1)在没有药物的情况下，再激活对冻结行为有显著影响——随着再激活时间从30秒增加到120秒，冻结行为和可能的恐惧诱发的应激增加；2)在再激活30秒（轻度应激）或120秒（强烈应激）后立即服用纳洛酮，在第二天分别增强或损害保留率。在第二个实验中，纳洛酮和ß-肾上腺素受体阻滞剂心得安分别或在120秒（强应激）激活后立即联合使用。结果表明，普萘洛尔和纳洛酮单独给药会损害保留率，而联合给药则不会。综上所述，这两个实验的结果与一种保护机制是一致的，这种机制是由内源性阿片和肾上腺素能系统协同作用介导的，它以压力依赖的方式在重新激活过程中限制了新获得的恐惧记忆的增强和损害。
Recent studies on the effect of stress on modulation of fear memory in our laboratory have uncovered endogenous opioid and adrenergic based modulation systems, working in concert, that limit the strengthening or weakening of newly acquired fear memory during consolidation under conditions of mild or intense stress, respectively. The present study sought to determine if similar stress-dependent modulation, mediated by endogenous opioid and adrenergic systems, occurs during reconsolidation of newly retrieved fear memory. Rats underwent contextual fear conditioning followed 24 hr later by reactivation of fear memory; a retention test was administered the next day. Stress was manipulated by varying duration of recall of fear memory during reactivation. In the first experiment, vehicle or the opioid-receptor blocker naloxone was administered immediately after varied durations (30 or 120 sec) of reactivation. The results indicate that 1) reactivation, in the absence of drug, has a marked effect on freezing behavior—as duration of reactivation increases from 30 to 120 sec, freezing behavior and presumably fearinduced stress increases and 2) naloxone, administered immediately after 30 sec (mild stress) or 120 sec (intense stress) of reactivation, enhances or impairs retention, respectively, the next day. In the second experiment, naloxone and the ß-adrenergic blocker propranolol were administered either separately or in combination immediately after 120 sec (intense stress) reactivation. The results indicate that separate administration of propranolol and naloxone impairs retention, while the combined administration fails to do so. Taken together the results of the two experiments are consistent with a protective mechanism, mediated by endogenous opioid and adrenergic systems working in concert, that limits enhancement and impairment of newly retrieved fear memory during reactivation in a stress-dependent manner.
登录
查看更多内容
DOI：
10.1523/jneurosci.2122-11.2011
发表时间：
2011-10-05
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Schutsky K;Ouyang M;Castelino CB;Zhang L;Thomas SA
通讯作者：
Thomas SA
影响因子：
2
作者：
Milton, Amy L.;Lee, Jonathan L. C.;Everitt, Barry J.
通讯作者：
Everitt, Barry J.
影响因子：
64.8
作者：
Nader, K;Schafe, GE;Le Doux, JE
通讯作者：
Le Doux, JE
影响因子：
5.3
作者：
Meilandt, WJ;Barea-Rodriguez, E;Martinez, JL
通讯作者：
Martinez, JL
DOI：
10.3758/bf03205285
发表时间：
1990-08-01
期刊：
ANIMAL LEARNING & BEHAVIOR
影响因子：
--
作者：
FANSELOW, MS
通讯作者：
FANSELOW, MS