Spatially controlled photothermal heating of bladder tissue through single-walled carbon nanohorns delivered with a fiberoptic microneedle device.
Spatially controlled photothermal heating of bladder tissue through single-walled carbon nanohorns delivered with a fiberoptic microneedle device.
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DOI：
10.1007/s10103-012-1202-4
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发表时间：
2013-07
影响因子：
2.1
通讯作者：
Rylander CG
中科院分区：
文献类型：
--
作者：
Hood RL;Carswell WF;Rodgers A;Kosoglu MA;Rylander MN;Grant D;Robertson JL;Rylander CG
基于激光的尿路上皮细胞癌（UCC）光热疗法仅限于浅表肿瘤的热消融，因为常用治疗波长下膀胱组织的浅光穿透阻碍了侵袭性病变的治疗。本研究评估了利用锋利的二氧化硅光纤微针装置 (FMD) 提供单壁碳纳米角 (SWNH) 作为外源发色团，与 1064 nm 激光结合使用，以空间控制的方式放大热处理剂量。进行实验以确定 SWNH 在水溶液 (0.05 mg/mL) 中的横向和深度扩散，该水溶液通过 FMD 注入健康、膨胀的离体猪膀胱壁。用自由空间、CW、1064 nm 激光照射 SWNH 灌注膀胱区域，以确定 SWNH 作为器官内外源生色团的功效。以 50 μL/min 的速率注入 SWNH 导致平均横向扩张率为 0.36 ± 0.08 cm2/min。对于 50 μL/min 输注 10 分钟或更短时间，输注的 SWNH 分散深度仅限于尿路上皮和肌肉固有层，但在 15 分钟输注时间后，分散到整个厚度。用 0.95 W/cm2 的 1064 nm 激光照射 SWNH 灌注的膀胱组织超过 40 秒，与非灌注对照中约 3°C 的增加相比，显示出约 19°C 的最大增加。结果表明，这些二氧化硅 FMD 可以成功地渗透到膀胱壁中，在一定程度的横向和深度控制下快速分布 SWNH，并且 SWNH 可能是用于基于激光的 UCC 治疗的光热放大的可行的外源生色团。
Laser-based photothermal therapies for urothelial cell carcinoma (UCC) are limited to thermal ablation of superficial tumors, as treatment of invasive lesions is hampered by shallow light penetration in bladder tissue at commonly used therapeutic wavelengths. This study evaluates the utilization of sharp, silica, fiberoptic microneedle devices (FMDs) to deliver single-walled carbon nanohorns (SWNHs) serving as exogenous chromophores in conjunction with a 1064 nm laser to amplify thermal treatment doses in a spatially controlled manner. Experiments were conducted to determine the lateral and depth dispersal of SWNHs in aqueous solution (0.05 mg/mL) infused through FMDs into the wall of healthy, inflated, ex vivo porcine bladders. SWNH perfused bladder regions were irradiated with a free-space, CW, 1064 nm laser in order to determine the SWNH efficacy as exogenous chromophores within the organ. SWNHs infused at a rate of 50 μL/min resulted in an average lateral expansion rate of 0.36 ± 0.08 cm2/min. Infused SWNHs dispersal depth was limited to the urothelium and muscular propria for 50 μL/min infusions of 10 minutes or less, but dispersed through the entire thickness after a 15 minute infusion period. Irradiation of SWNH perfused bladder tissue with 1064 nm laser light at 0.95 W/cm2 over 40 seconds exhibited a maximum increase of approximately 19°C compared with an increase of approximately 3°C in a non-perfused control. The results indicate that these silica FMDs can successfully penetrate into the bladder wall to rapidly distribute SWNHs with some degree of lateral and depth control and that SWNHs may be a viable exogenous chromophore for photothermal amplification of laser-based UCC treatments.
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影响因子：
2.4
作者：
JOHNSON, DE
通讯作者：
JOHNSON, DE
影响因子：
2.8
作者：
Iijima, S;Yudasaka, M;Takahashi, K
通讯作者：
Takahashi, K
DOI：
10.1152/ajpregu.1999.277.4.r1218
发表时间：
1999-10-01
影响因子：
2.8
作者：
Morrison, PF;Chen, MY;Oldfield, EH
通讯作者：
Oldfield, EH
DOI：
10.1016/0006-291x(73)91046-2
发表时间：
1973-01-01
影响因子：
3.1
作者：
BRONK, BV;WILKINS, RJ;REGAN, JD
通讯作者：
REGAN, JD
影响因子：
--
作者：
Dowlatshahi, K;Fan, M;Ali, A
通讯作者：
Ali, A