Telomere dynamics in wild banded mongooses: Evaluating longitudinal and quasi-longitudinal markers of senescence.
Telomere dynamics in wild banded mongooses: Evaluating longitudinal and quasi-longitudinal markers of senescence.
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DOI：
10.1016/j.exger.2017.09.021
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发表时间：
2018-07-01
影响因子：
3.9
通讯作者：
Cant MA
中科院分区：
文献类型：
--
作者：
Hares MC;Vitikainen EIK;Marshall HH;Thompson FJ;Blount JD;Cant MA
端粒长度和端粒缩短速率被认为是与躯体和生殖功能衰老有关的过程中特别有用的生理生物标志物。然而，很难获得关于整个寿命过程中端粒长度变化的纵向数据，特别是对于长寿的动物。准纵向研究已经被提出作为一种方法来深入了解长寿物种的端粒动态。在这种方法中，最小复制细胞被用作基线端粒长度，与高复制细胞(代表当前状态)的端粒长度进行比较。在这里，我们使用野生合作哺乳动物Mungos Mungo的纵向端粒数据来测试准纵向方法的假设和预测。与我们的预测相反，白细胞的端粒长度(TL)长于耳软骨。纵向上，耳软骨的热释光随年龄的增长而缩短，而白细胞的热释光没有变化，我们还观察到许多个体的热释光随年龄增长而不是减少。白细胞TL而不是软骨TL是总寿命的预测因子，而两者都不能预测采样后的存活率。我们的数据不支持TL的跨组织比较可以作为衰老的准纵向标记的假设。相反，我们的结果表明，带状猫鼬的端粒动力学比通常假设的要复杂得多，需要进行贯穿整个生命周期的纵向研究，以阐明端粒动力学与自然种群衰老之间的联系。我们没有发现体细胞组织可以作为白细胞端粒长度的准纵向标记的证据。不同组织类型的端粒动力学似乎是复杂的，并且可能受到端粒酶活性的影响。端粒长度可能是野生动物种群体细胞质量的有用标记。
Telomere length and the rate of telomere shortening have been suggested as particularly useful physiological biomarkers of the processes involved in senescent decline of somatic and reproductive function. However, longitudinal data on changes in telomere length across the lifespan are difficult to obtain, particularly for long-lived animals. Quasi-longitudinal studies have been proposed as a method to gain insight into telomere dynamics in long-lived species. In this method, minimally replicative cells are used as the baseline telomere length against which telomere length in highly replicative cells (which represent the current state) can be compared. Here we test the assumptions and predictions of the quasi-longitudinal approach using longitudinal telomere data in a wild cooperative mammal, the banded mongoose, Mungos mungo. Contrary to our prediction, telomere length (TL) was longer in leukocytes than in ear cartilage. Longitudinally, the TL of ear cartilage shortened with age, but there was no change in the TL of leukocytes, and we also observed many individuals in which TL increased rather than decreased with age. Leukocyte TL but not cartilage TL was a predictor of total lifespan, while neither predicted post-sampling survival. Our data do not support the hypothesis that cross-tissue comparison in TL can act as a quasi-longitudinal marker of senescence. Rather, our results suggest that telomere dynamics in banded mongooses are more complex than is typically assumed, and that longitudinal studies across whole life spans are required to elucidate the link between telomere dynamics and senescence in natural populations. We find no evidence that somatic tissues can be used as a quasi-longitudinal marker for telomere length in leukocytes. Telomere dynamics in different tissue types appear to be complex and likely to be influenced by telomerase activity. Telomere length may be a useful marker for somatic quality in wild animal populations.
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