A Novel Volume-Stable Collagen Matrix Induces Changes in the Behavior of Primary Human Oral Fibroblasts, Periodontal Ligament, and Endothelial Cells.
A Novel Volume-Stable Collagen Matrix Induces Changes in the Behavior of Primary Human Oral Fibroblasts, Periodontal Ligament, and Endothelial Cells.
复制标题
DOI：
10.3390/ijms22084051
复制
发表时间：
2021-04-14
影响因子：
5.6
通讯作者：
Sculean A
中科院分区：
文献类型：
--
作者：
Asparuhova MB;Stähli A;Guldener K;Sculean A
本研究的目的是研究一种新型体积稳定胶原基质（vCM）对早期伤口愈合事件的影响，包括细胞迁移和粘附，蛋白质吸附和释放，以及止血系统的动力学。为此，我们使用了transwell迁移和结晶紫粘附实验，elisa用于定量基质上吸附和释放的生长因子，qRT-PCR用于定量基质上生长细胞的基因表达。我们的研究结果表明，与没有基质的对照细胞相比，原代人口腔成纤维细胞、牙周韧带和内皮细胞向vCM的迁移增加。与对照组相比，vCM的细胞粘附性能显著提高。生长因子TGF-β1、PDGF-BB、FGF-2和GDF-5被高效吸附在vCM上，并在数小时内初始爆发释放后持续递送至培养基中。我们观察到，在vCM上生长的细胞中，编码抗纤溶性血栓调节蛋白、纤溶酶原激活物抑制剂1型、血栓反应蛋白1和血栓活酶的基因显著上调，编码纤溶性原组织纤溶酶原激活物、尿激酶型纤溶酶原激活物及其受体和基质金属蛋白酶14的基因显著下调。总的趋势是，vCM对抗纤溶基因表达的刺激作用被TGF-β1、PDGF-BB或FGF-2协同增强，而vCM对抗纤溶基因表达的强抑制作用被PDGF-BB、FGF-2或GDF-5逆转。综上所述，我们的数据有力地支持了新型vCM对纤维蛋白凝块稳定和凝血/纤维蛋白溶解平衡的影响，从而促进了软组织愈合过程的下一阶段的进展。
The aim of the present study was to investigate the influence of a novel volume-stable collagen matrix (vCM) on early wound healing events including cellular migration and adhesion, protein adsorption and release, and the dynamics of the hemostatic system. For this purpose, we utilized transwell migration and crystal violet adhesion assays, ELISAs for quantification of adsorbed and released from the matrix growth factors, and qRT-PCR for quantification of gene expression in cells grown on the matrix. Our results demonstrated that primary human oral fibroblasts, periodontal ligament, and endothelial cells exhibited increased migration toward vCM compared to control cells that migrated in the absence of the matrix. Cellular adhesive properties on vCM were significantly increased compared to controls. Growth factors TGF-β1, PDGF-BB, FGF-2, and GDF-5 were adsorbed on vCM with great efficiency and continuously delivered in the medium after an initial burst release within hours. We observed statistically significant upregulation of genes encoding the antifibrinolytic thrombomodulin, plasminogen activator inhibitor type 1, thrombospondin 1, and thromboplastin, as well as strong downregulation of genes encoding the profibrinolytic tissue plasminogen activator, urokinase-type plasminogen activator, its receptor, and the matrix metalloproteinase 14 in cells grown on vCM. As a general trend, the stimulatory effect of the vCM on the expression of antifibrinolytic genes was synergistically enhanced by TGF-β1, PDGF-BB, or FGF-2, whereas the strong inhibitory effect of the vCM on the expression of profibrinolytic genes was reversed by PDGF-BB, FGF-2, or GDF-5. Taken together, our data strongly support the effect of the novel vCM on fibrin clot stabilization and coagulation/fibrinolysis equilibrium, thus facilitating progression to the next stages of the soft tissue healing process.
登录
查看更多内容
影响因子：
20.3
作者：
Aburima, Ahmed;Berger, Martin;Naseem, Khalid M
通讯作者：
Naseem, Khalid M
影响因子：
6.9
作者：
Belotti, Dorina;Capelli, Chiara;Taraboletti, Giulia
通讯作者：
Taraboletti, Giulia
影响因子：
7.6
作者：
Cochran, D. L.;Oh, T. -J.;Takemura, A.
通讯作者：
Takemura, A.
影响因子：
4.3
作者：
Cieslik-Wegemund, Marta;Wierucka-Mynarczyk, Beata;Gilowski, Lukasz
通讯作者：
Gilowski, Lukasz
影响因子：
3.4
作者：
Caballe-Serrano, Jordi;Zhang, Sophia;Bosshardt, Dieter D.
通讯作者：
Bosshardt, Dieter D.