B10 cells induced by Schistosoma japonicum soluble egg antigens modulated regulatory T cells and cytokine production of T cells
B10 cells induced by Schistosoma japonicum soluble egg antigens modulated regulatory T cells and cytokine production of T cells
复制标题
日本血吸虫可溶性卵抗原诱导的 B10 细胞调节调节性 T 细胞和 T 细胞的细胞因子产生
DOI：
10.1007/s00436-015-4613-x
复制
发表时间：
2015-07
影响因子：
2
通讯作者：
Qian Li
中科院分区：
文献类型：
--
作者：
Tian Fang;Hu Xueli;Xian Kangwen;Zong Dayuan;Liu Hao;Wei Hui;Yang Weiping;Qian Li
A distinct subset of B cells, also known as regulatory B cells, can negatively regulate T cell immune responses, but the role of these cells in schistosomiasis has not been clarified. Soluble egg antigen (SEA) and soluble adult worm antigen preparation (SWAP), which are two important antigen sources during Schistosoma japonicum infection, both can induce Th1, Th2, Th17, and Treg cells and the corresponding cytokines. However, whether they can induce the production of regulatory B cells and the regulatory function of schistosome-induced regulatory B cells remains unclear. In our studies, we first analyzed the production of regulatory B cells stimulated by SEA or SWAP using flow cytometry and enzyme-linked immunosorbent assay, and observed these cells in mice immunized by SEA or SWAP. Then, B10 cells sorted by MicroBeads were co-cultured with CD4(+) T cells, and the proportion of Treg cells were detected. At the same time, the IFN-γ, IL-4, and IL-17 levels in the culture supernatant were measured. The results showed that B10 cells were preferentially induced by SEA in vitro, and B10 could also be induced in mice immunized by SEA. SEA-induced B10 cells promoted the expansion of regulatory T cells and induced IL-4 secretion, but inhibited IL-17 production. These findings reveal that the generation of B10 cells is determined by parasitic antigen, and suggest the function of B10 cell induced by SEA. This study significantly contributes to the understanding of the immune regulatory role in schistosomiasis and may help protect hosts from infection.
登录
查看更多内容
影响因子：
3.7
作者：
van der Vlugt LE;Labuda LA;Ozir-Fazalalikhan A;Lievers E;Gloudemans AK;Liu KY;Barr TA;Sparwasser T;Boon L;Ngoa UA;Feugap EN;Adegnika AA;Kremsner PG;Gray D;Yazdanbakhsh M;Smits HH
通讯作者：
Smits HH
影响因子：
4.4
作者：
Mangan, NE;Fallon, RE;Fallon, PG
通讯作者：
Fallon, PG
DOI：
10.4049/jimmunol.0900270
发表时间：
2009-06-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Yanaba K;Bouaziz JD;Matsushita T;Tsubata T;Tedder TF
通讯作者：
Tedder TF
影响因子：
5.4
作者：
Bouma, Gerben;Carter, Natalie A.;Recher, Mike;Malinova, Dessislava;Adriani, Marsilio;Notarangelo, Luigi D.;Burns, Siobhan O.;Mauri, Claudia;Thrasher, Adrian J.
通讯作者：
Thrasher, Adrian J.
影响因子：
4.4
作者：
P. Velupillai;W. Secor;A. Horauf;D. Harn
通讯作者：
P. Velupillai;W. Secor;A. Horauf;D. Harn