Molecular characterization and bioactivity evaluation of two novel bombinin peptides from the skin secretion of Oriental fire-bellied toad, Bombina orientalis
Molecular characterization and bioactivity evaluation of two novel bombinin peptides from the skin secretion of Oriental fire-bellied toad, Bombina orientalis
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东方火腹蟾蜍皮肤分泌物中两种新型铃蟾肽的分子表征和生物活性评价
DOI：
10.1007/s00726-017-2509-z
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发表时间：
2017-11
期刊：
影响因子：
3.5
通讯作者：
Kong Dexin
中科院分区：
文献类型：
--
作者：
Peng Xin;Zhou Chang;Hou Xuan;Liu Yao;Wang Zhengming;Peng Xiaolin;Zhang Zhe;Wang Ran;Kong Dexin
随着对两栖动物防御性皮肤分泌物中生物化学物质的探索，具有独特分子结构和复杂特征与功能的新型生物活性肽受到了人们的广泛关注。本研究以东方火腹蟾蜍（Bombina orientalis）的皮肤分泌物为材料，寻找具有治疗作用的多肽。利用鸟枪法克隆技术，从B的皮肤分泌物cDNA文库中克隆到了一个共编码两个新的铃蟾肽的全长肽前体。东方结果表明，所推导的肽段分别为一个bombinin-like peptide（BLP）（GIGSAILSAGKSIIKGLAKGLAEHF-NH 2）和一个bombinin-H型肽（BH）（IIGPVLGLVGKALGGLL-NH 2）。两种肽的一级结构通过反相HPLC分馏和质谱法确认。二级结构预测表明Bombinin-BO 1和BombininH-BO 1具有α-螺旋结构特征。此外，这两种多肽对革兰氏阳性菌、革兰氏阴性菌和酵母菌均表现出广谱抗菌作用。同时，抗癌活性测定表明两种肽对测试的人肝癌细胞系（Hep G2/SK-HEP-1/Huh 7）具有显著的抗癌作用。本文首次报道的肽可能代表用于设计/开发人类感染和肿瘤疾病的新疗法的新型先导化合物。
Following the exploration of biochemicals in amphibian defensive skin secretion, great attention has been focused on the novel bioactive peptides with unique molecular structures and complicated features and functions. In this study, the skin secretion of Oriental fire-bellied toad, Bombina orientalis, was acquired to search peptides with therapeutic potential. Using “shotgun” cloning technique, a full-length peptide precursor co-encoding two novel bombinin peptides was cloned from the skin secretion-derived cDNA library of B. orientalis. The deduced peptides were identified as one bombinin-like peptide (BLP) (GIGSAILSAGKSIIKGLAKGLAEHF-NH2) and one bombinin H-type peptide (BH) (IIGPVLGLVGKALGGLL-NH2). The primary structures of both peptides were confirmed through reverse-phase HPLC fractionation and mass spectrometry. Secondary structural prediction revealed Bombinin-BO1 and Bombinin H-BO1 adopted α-helical structural features. In addition, the two peptides exhibited broad-spectrum antimicrobial effect against Gram-positive and Gram-negative bacteria and yeast. Meanwhile, the anticancer activity assay indicated both peptides exerted significant anticancer effects against human hepatoma cell lines tested (Hep G2/SK-HEP-1/Huh7). The peptides reported here for the first time may represent novel lead compounds for the design/development of new therapeutics for human infection and neoplastic disease.
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影响因子：
4.2
作者：
Wan Y;Ma C;Zhou M;Xi X;Li L;Wu D;Wang L;Lin C;Lopez JC;Chen T;Shaw C
通讯作者：
Shaw C
影响因子：
3.8
作者：
C. Stevens;G. Tóth;A. Borsodi;S. Benyhe
通讯作者：
C. Stevens;G. Tóth;A. Borsodi;S. Benyhe
DOI：
10.1016/s0021-9258(18)54469-0
发表时间：
1991-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
B. Gibson;D. Tang;R. Mandrell;M. Kelly;E. Spindel
通讯作者：
B. Gibson;D. Tang;R. Mandrell;M. Kelly;E. Spindel
DOI：
10.1002/chin.200637243
发表时间：
2006-09
期刊：
ChemInform
影响因子：
--
作者：
T. Pukala;J. Bowie;V. M. Maselli;I. Musgrave;M. Tyler
通讯作者：
T. Pukala;J. Bowie;V. M. Maselli;I. Musgrave;M. Tyler
影响因子：
11.4
作者：
MIGNOGNA, G;SIMMACO, M;BARRA, D
通讯作者：
BARRA, D