The evolution of tachykinin/tachykinin receptor (TAC/TACR) in vertebrates and molecular identification of the TAC3/TACR3 system in zebrafish (Danio rerio)
The evolution of tachykinin/tachykinin receptor (TAC/TACR) in vertebrates and molecular identification of the TAC3/TACR3 system in zebrafish (Danio rerio)
复制标题
脊椎动物中速激肽/速激肽受体（TAC/TACR）的进化以及斑马鱼（Danio rerio）TAC3/TACR3系统的分子鉴定
DOI：
10.1016/j.mce.2012.04.007
复制
发表时间：
2012-09
影响因子：
4.1
通讯作者：
Lin, Haoran
中科院分区：
文献类型：
--
作者：
Zhou, Wenyi;Li, Shuisheng;Liu, Yun;Qi, Xin;Chen, Huapu;Cheng, Christopher H. K.;Liu, Xiaochun;Zhang, Yong;Lin, Haoran
速激肽是从无脊椎动物到哺乳动物保守的肽家族。然而，对脊椎动物，特别是硬骨鱼类中速激肽（TAC）和速激肽受体（TACR）基因的进化历史却知之甚少。在本研究中，在斑马鱼基因组中鉴定了5个TAC和6个TACRs基因。基因组同线性分析和系统发育树分析表明，脊椎动物TAC和TACR基因数量的增加是基因组重复和局部个体基因重复的结果。随后从斑马鱼脑样品中克隆了编码多个TAC 3s（TAC 3a和TAC 3b）和TACR 3s（TACR 3a 1、TACR 3a 2和TACR 3b）的全长cDNA序列。序列分析表明，斑马鱼TAC 3前体蛋白经过加工后，可能产生4个神经激肽B（NKB）样肽（NKBa-13、NKBa-10、NKB B-13和NKB B-11）。在斑马鱼中的组织分布研究表明，TAC 3和TACR 3主要在脑区域表达。在培养的真核细胞中使用转录报告基因分析进一步检测了四种斑马鱼NKB肽和三种TACR 3的生物学活性。除了NKBb-11之外，所有合成的NKB肽都能够引起TACR 3的下游信号传导事件。这些结果表明，在脊椎动物中鉴定的多个TAC/TACR基因是从基因复制事件进化而来的，并且TAC 3/TACR 3系统也在硬骨鱼类中起作用。
Tachykinins are a family of peptides that are conserved from invertebrates to mammals. However, little is known about the evolutionary history of tachykinin (TAC) and tachykinin receptor (TACR) genes in vertebrates, especially in the teleost group. In the present study, five TACs and six TACRs genes were identified in the zebrafish genome. Genomic synteny analysis and phylogenetic tree analysis indicate that the increased numbers of TAC and TACR genes in vertebrates are the result of both genome duplications and local individual gene duplication. The full-length cDNA sequences encoding multiple TAC3s (TAC3a and TAC3b) and TACR3s (TACR3a1, TACR3a2 and TACR3b) were subsequently cloned from zebrafish brain samples. Sequence analysis suggested that four putative neurokinin B (NKB)-like peptides (NKBa-13, NKBa-10, NKBb-13 and NKBb-11) might be generated by the processing of two zebrafish TAC3 precursors. Tissue distribution studies in zebrafish revealed that TAC3 and TACR3 are mainly expressed in the brain regions. The biological activities of four zebrafish NKB peptides and three TACR3s were further examined using transcription reporter assays in cultured eukaryotic cells. All the synthetic NKB peptides were able to evoke the downstream signaling events of TACR3s with the exception of NKBb-11. These results indicated that the multiple TAC/TACR genes identified in vertebrates evolved from gene duplication events and that the TAC3/TACR3 systems also operate in the teleost group.
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DOI：
10.1523/jneurosci.10-07-02203.1990
发表时间：
1990-07
期刊：
--
影响因子：
--
作者：
M. Carter;J. Krause
通讯作者：
M. Carter;J. Krause
DOI：
10.1016/0006-291x(91)91704-g
发表时间：
1991-09
影响因子：
3.1
作者：
Yasuo Takeda;K. B. Chou;J. Takeda;B. Sachais;J. E. Krause
通讯作者：
Yasuo Takeda;K. B. Chou;J. Takeda;B. Sachais;J. E. Krause
影响因子：
14.9
作者：
Southey BR;Amare A;Zimmerman TA;Rodriguez-Zas SL;Sweedler JV
通讯作者：
Sweedler JV
影响因子：
3.5
作者：
Kurtz, MM;Wang, RP;Liu, QY
通讯作者：
Liu, QY
DOI：
10.1016/s0021-9258(18)53041-6
发表时间：
1993-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
U. Gether;T. Johansen;T. Schwartz
通讯作者：
U. Gether;T. Johansen;T. Schwartz